Cd41 bv421

Manufactured by BD

The CD41 BV421 is a flow cytometry reagent used for the detection and analysis of CD41 expression on cells. It is a fluorochrome-conjugated antibody that binds to the CD41 antigen, which is a marker expressed on the surface of platelets. The CD41 BV421 reagent can be used in a variety of applications, such as immunophenotyping and cell sorting.

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Lab products found in correlation

Siglec f bv421, by BD (2 mentions) Cd64 pe cy7, by BioLegend (2 mentions) Cd115 alexa fluor 488, by BioLegend (2 mentions) Cd93 percp cy5, by BioLegend (2 mentions) F4 80 pe cf594, by BD (2 mentions) Sca 1 allophycocyanin, by BioLegend (2 mentions) Cd16 32 allophycocyanin r700, by BD (2 mentions) Cd11c allophycocyanin 780, by Thermo Fisher Scientific (2 mentions) Ter119 bv510, by BD (2 mentions) Cx3cr1 bv650, by BioLegend (2 mentions) C kit bv711, by BD (2 mentions) Cd3e buv395, by BD (2 mentions) Cd11b buv737, by BD (2 mentions) Vcam1 alexa fluor 647, by BioLegend (2 mentions) Mhc class 2 alexa fluor 700, by Thermo Fisher Scientific (2 mentions) Tim 4 bv510, by BD (2 mentions) Cd45 bv786, by BD (2 mentions) Cd144 pe, by BD (2 mentions) Ly6g bv605, by BD (2 mentions) Cd68 percp cy5, by BioLegend (2 mentions)

3 protocols using cd41 bv421

Comprehensive Flow Cytometry Panel

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The following Abs were used for flow cytometry: CD115 Alexa Fluor 488 (AFS98, BioLegend, 135512), CD93 PerCP-Cy5.5 (AA4.1, BioLegend, 136512), CD144 PE (11D4.1, BD Biosciences, 562243), F4/80 PE-CF594 (T45-2342, BD Biosciences, 565613), CD64 PE-Cy7 (X54-5/7.1, BioLegend, 139314), Sca-1 allophycocyanin (D7, BioLegend, 108112), CD16/32 allophycocyanin-R700 (2.4G2, BD Biosciences, 565502), CD11c allophycocyanin-780 (N418, eBio-science, 47011482), CD41 BV421 (MWReg30, BD Biosciences, 747729), Ter119 BV510 (TER-119, BD Biosciences, 563995), Ly6G BV605 (1A8, BD Biosciences, 563005), CX3CR1 BV650 (SA011F11, BioLegend, 149033), c-Kit BV711 (2B8, BD Biosciences, 105835), CD45 BV786 (30-F11, BD Biosciences, 564225), CD3e BUV395 (145-2C11, BD Biosciences, 563565), CD11b BUV737 (M1/70, BD Biosciences, 564443), CD68 PerCP-Cy5.5 (FA-11, BioLegend, 137010), VCAM1 Alexa Fluor 647 (429, BioLegend, 105712), MHC class II Alexa Fluor 700 (M5/114, Thermo Fisher Scientific, 56532182), Siglec-F BV421 (E50-2440, BD Biosciences, 562681), Tim-4 BV510 (21H12, BD Biosciences, 742774), Live/Dead, fixable UV (Thermo Fisher Scientific, L34962).

Philpott J., Kazimierczyk S., Korgaonkar P., Bordt E., Zois J., Vasudevan C., Meng D., Bhatia I., Lu N., Jimena B., Porter C., Cherayil B.J, & Jain N. (2022). RXRα Regulates the Development of Resident Tissue Macrophages. ImmunoHorizons, 6(6), 366-372.

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Comprehensive Flow Cytometry Panel

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Peripheral Blood Cell Profiling by Flow Cytometry

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Presence of various human cells in the peripheral blood was detected by flow cytometry as described earlier31 (link). Various antibodies, including CD3 PE Cy7 (#555349), CD4 APC (#555349), CD8 PE (#555635), CD14 APC H7 (#561384), CD19 BV510 (#562953) and CD41 BV421 (#563312) were obtained from BD Biosciences (San Jose, CA). The cells were acquired using LSR II Flow Cytometer (BD Biosciences). Cells stained with only CD3, only CD14 and unstained cells were used as controls. 15000 events were acquired per sample in the leukocyte gate based on forward and side scatter. All CD14⁺ cells, which also expressed CD41, a platelet marker, were characterized as platelet monocyte complexes. The percentage of the individual cell types against total leukocyte population was numerically used to plot the graphs shown in Fig. 1.

Singh V.B., Singh M.V., Gorantla S., Poluektova L.Y, & Maggirwar S.B. (2016). Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice. Scientific Reports, 6, 26876.

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