Total protein was extracted from cells and tissues with RIPA lysis buffer
(Invitrogen) and western blot was conducted using standard methods as reported.20 (link) Primary antibodies including anti-BCL2 associated X (anti-bax, ab262929;
dilution 1:1,000), anti-proliferating cell nuclear antigen (anti-PCNA, ab220208;
dilution 1:1,000), anti-P-glycoprotein (anti-P-pg, ab235954; dilution 1:1,000),
anti-multidrug resistance-associated protein 1 (anti-MRP1, ab32574; dilution
1:500), anti-CORO1C (ab96266; dilution 1:2,000) and
anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH, ab157156; dilution
1:1,000) were used as recommended by the manufacturers (Abcam, Cambridge, UK).
Anti-rabbit and anti-mouse IgG (ab205718 and ab97046, Abcam; dilution 1:10,000
and 1:10,000) labeled by horseradish peroxidase were used as secondary
antibodies. Immunoreactive bands were developed with a Chemiluminescent Kit
(Bio-Rad, Kidlington, UK).
(Invitrogen) and western blot was conducted using standard methods as reported.20 (link) Primary antibodies including anti-BCL2 associated X (anti-bax, ab262929;
dilution 1:1,000), anti-proliferating cell nuclear antigen (anti-PCNA, ab220208;
dilution 1:1,000), anti-P-glycoprotein (anti-P-pg, ab235954; dilution 1:1,000),
anti-multidrug resistance-associated protein 1 (anti-MRP1, ab32574; dilution
1:500), anti-CORO1C (ab96266; dilution 1:2,000) and
anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH, ab157156; dilution
1:1,000) were used as recommended by the manufacturers (Abcam, Cambridge, UK).
Anti-rabbit and anti-mouse IgG (ab205718 and ab97046, Abcam; dilution 1:10,000
and 1:10,000) labeled by horseradish peroxidase were used as secondary
antibodies. Immunoreactive bands were developed with a Chemiluminescent Kit
(Bio-Rad, Kidlington, UK).