ALP activity was measured using a Lab assay ALP kit (#633–51021 FUJIFILM Wako) according to the manufacturer's instructions and normalized to total protein measured using the BCA protein assay kit. For mineralization, ST-2 cells were cultured in 96-well plates in the presence and absence of 200 ng/ml BMP-2, with and without 10 µg/mL Myo-EVs for 1 week, then the cells were cultured in mineralization media (low-glucose DMEM/10% FBS and 1% PS supplemented with 10 mM β-glycerophosphate and 50 µg/mL ascorbic acid) with and without 10 µg/mL Myo-EVs for more than 10 days. To evaluate mineralization levels, the cells were fixed with 10% formaldehyde and stained with Alizarin red (Kishida Chemical, Osaka, Japan). The cells were destained with cetylpyridinium chloride (Wako Pure Chemical) and the absorbance at 570 nm was measured by the Multiskan GO microplate spectrophotometer (Thermo Fisher Scientific).
Cetylpyridinium chloride
Manufactured by Fujifilm
Sourced in Japan
Cetylpyridinium chloride is a quaternary ammonium compound that functions as a disinfectant and antiseptic. It is commonly used in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Labassay alp kit, by Fujifilm (1 mentions)
Multiskan go microplate spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Alizarin red solution, by Merck Group (1 mentions)
Bz x800 microscope, by Keyence (1 mentions)
Pyrene, by Fujifilm (1 mentions)
Tetrahydrofuran thf, by Fujifilm (1 mentions)
Ethanol, by Fujifilm (1 mentions)
2 methoxyethyl acrylate mea, by Fujifilm (1 mentions)
Tris 2 dimethylamino ethyl amine me6tren, by Merck Group (1 mentions)
Benzalkonium chloride, by Fujifilm (1 mentions)
Sodium lauryl sulfate, by Fujifilm (1 mentions)
4 protocols using cetylpyridinium chloride
1
Evaluating Osteoblast Mineralization Induced by Myo-EVs
2
Osteogenic Differentiation of VICs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normo-VICs and hypo-VICs were plated onto 96-well plates at 80% confluency, in DMEM or DMEM-based osteogenic medium (OGM) supplemented with Osteoblast-Inducer Reagent under normoxia for three weeks. Medium was changed every 7 d. Calcification of VICs was visualized using 1% Alizarin Red solution (Sigma-Aldrich) after fixation with methanol. Micrographs were captured using a BZ-X800 microscope (Keyence, Kyoto, Japan). Quantification was performed by measuring the absorbance (560 nm) of 100 nM of cetylpyridinium chloride (Wako, Japan) extracted from the stained VICs.
3
Synthesis and Characterization of PEO-Br Macroinitiator
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tris(2-(dimethylamino)ethyl)amine (Me6TREN, 97%) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Copper bromide (CuBr, 95.0%) was supplied by Kishida Chemical (Osaka, Japan). Ethanol (99.5%), tetrahydrofuran (THF, 99.5%), and cetylpyridinium chloride (90%) were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). All chemicals were used as received; 2-Methoxyethyl acrylate (MEA, 98.0%) from Fujifilm Wako Pure Chemical (Osaka, Japan) was treated with an inhibitor-remover prepacked column from Sigma-Aldrich (St. Louis, MO, USA) prior to use. PEO (MW = 500,000 g/mol) was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). PEO-based macroinitiator (PEO-Br) was prepared and purified in accordance with the literature [28 (link)]. Number average molecular weight (Mn(GPC)) and molecular weight distribution (Mw/Mn) estimated from gel-permeation chromatography (GPC) for PEO-Br were 4.64 × 105 g/mol and 1.23, respectively. The degree of polymerization (DP) for PEO-Br was 11,340. Pyrene (97%) from Fujifilm Wako Pure Chemical (Osaka, Japan) was recrystallized from mEthanol. Water was purified using an ion-exchange column system.
4
Surfactant Comparison for Cosmetic Applications
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Benzalkonium chloride (BC), cetylpyridinium chloride (CPC), sodium lauryl sulfate (SLS), and lauryl betaine (LB, 35% lauryl dimethylaminoacetic acid solution) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), and lautrimonium chloride (LC, lauryltrimethylammonium chloride) is a raw material used in cosmetics. Therefore, we used reagents that differed from those used in the previous study (Table 1).
Additional surfactants used were sodium laurate (SL), oleth 10 (OL, Polyoxyethylene [10] oleyl ether), and sodium lauryl glutamate (SLG). SL and OL were purchased from FUJIFILM Wako Pure Chemical Corporation. SLG is a raw material used in cosmetics (Table 2).
Additional surfactants used were sodium laurate (SL), oleth 10 (OL, Polyoxyethylene [10] oleyl ether), and sodium lauryl glutamate (SLG). SL and OL were purchased from FUJIFILM Wako Pure Chemical Corporation. SLG is a raw material used in cosmetics (Table 2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!