Samples were characterized for molecular alterations by targeted NGS (Ion Ampliseq Colon-Lung Cancer Research Panel V2, Life Technologies). Briefly the multiplex barcoded libraries are generated from 10 ng of DNA following manufacturer’s recommendations (Ion Ampliseq Library Kit V2) and are normalized using the Ion Library Equalizer Kit. The pooled libraries (max 96) are processed on Ion Chef System for template preparation and chip loading (Ion Pi Hi-Q Chef Kit, Ion Pi Chip V3), and sequenced on the Ion Proton System (Life Technologies). The FASTQs sequencing data are aligned to the human genome (hg19) and processed using Iontorrent Suite V5.0.4.0 This package included the Torrent Variant Caller V5.0.4.0 using the built-in “Somatic - low stringency” with optimized parameters to automatically call variants with allelic ratio>2%.
Ion ampliseq colon lung cancer research panel v2
Manufactured by Thermo Fisher Scientific
The Ion AmpliSeq Colon and Lung Cancer Research Panel v2 is a targeted sequencing panel designed for cancer research. It enables the simultaneous analysis of 22 genes associated with colon and lung cancers. The panel leverages Ion AmpliSeq technology to provide a streamlined workflow for library preparation and sequencing on Ion Torrent next-generation sequencing systems.
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Lab products found in correlation
2 protocols using ion ampliseq colon lung cancer research panel v2
1
Targeted NGS for Cancer Mutation Profiling
2
Targeted NGS for Molecular Alterations
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Samples were characterized for molecular alterations by targeted next-generation sequencing (NGS) (Ion AmpliSeq™ Colon-Lung Cancer Research Panel v2, Life Technologies™, Carlsbad, CA) as previously described [32 (link)].
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