TUEP and UEP were diluted 10 times with PBS buffer, then the mixture was homogenized and centrifuged, the protein concentration of supernatant was diluted to 0.3 mg/mL. Then, 2 mL of diluted sample was mixed with 40 μL ANS solution (sodium 8-anilino-1-naphthalenesulfonate dissolved in ethanol, 1 mmol/L) and store in the dark for 10 min. Finally, the sample was analyzed for surface hydrophobicity using a Hitachi F-7000 Flourescence Spectrofluorometer (Hitachi, Tokyo, Japan) at the emission wavelength and excitation wavelength of 485 nm and 380 nm [17] .
F 7000 flourescence spectrofluorometer
Manufactured by Hitachi
Sourced in Japan
The F-7000 Fluorescence Spectrofluorometer is a laboratory instrument designed to measure the fluorescence properties of samples. It is capable of detecting and analyzing the emission spectra of fluorescent materials, which can provide valuable information about the chemical composition and structure of the sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using f 7000 flourescence spectrofluorometer
1
Measuring Protein Surface Hydrophobicity
2
Protein Surface Hydrophobicity Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The gels were diluted tenfold with PBS buffer, followed by homogenization and centrifugation. The protein concentration of the supernatant was adjusted to 0.3 mg/mL. Subsequently, 2 mL of the diluted sample was combined with 40 μL of ANS solution (sodium 8-anilino-1-naphthalenesulfonate dissolved in ethanol, 1 mmol/L) and incubated in the dark for 10 min. Finally, surface hydrophobicity analysis was performed using a Hitachi F-7000 Flourescence Spectrofluorometer (Hitachi, Tokyo, Japan) with excitation and emission wavelengths set at 380 nm and 485 nm, respectively [23 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!