Limonene

(E)-6,6′-Dibromo-[3,3′-diindolylidene]-2,2′-dione (97%), benzo [1,2-b:4,5-b′]dithiophene-4,8-dione (98%), trimethyltin chloride (99%, 1.0 mol/L in hexane), n-butyllithium (97%, 2.5 mol/L in hexane), sodium hydroxide (97%), zinc powder (98%, 200 mesh), diethylene glycol monomethyl ether (98%), triethylene glycol monomethyl ether (99%), tetraethyleneglycol monomethyl ether (98%), 2-hexyl-1-decanol (94%), methyl sulfonyl chloride (99%), tris(dibenzylideneacetone)dipalladium chloroform adduct (99%), tris(o-methylphenyl)phosphine (99%), tetrahydrofuran (99%, extra dry, with molecular sieves, water ≤ 50 ppm), o-xylene (99%, extra dry, with molecular sieves, water ≤ 50 ppm), tetrabutylammonium hexafluorophosphonate (99%), acetonitrile (99%, extra dry, with molecular sieves, water ≤ 50 ppm), and limonene (99%) were purchased from Energy Chemical Company (Anqing, China) and were not treated in any way prior to use. Other solvents, such as 2-methyltetrahydrofuran, ethyl acetate, and trichloromethane are analytically pure and purchased from Sinopharm (Shanghai, China).

(−)-Limonene, (−)-perillyl alcohol and (−)-perillyl aldehyde were purchased from Energy chemical Ltd. (Shanghai, China). All other chemicals of analytical grade or higher purity were obtained from commercial sources without further purification. Tryptone and yeast extract were obtained from Oxoid (Hampshire, UK). Genes were synthesized by Genscript Biotech Co. Ltd. (Nanjing, China). Formate dehydrogenase from Candida boidinii (CbFDH), alcohol dehydrogenase from Lactobacillus kefiri (LkADH), NADH oxidase from Streptococcus mutans (SmNOX) and w-transaminase from Arthrobacter sp. (ATA-117) were obtained from the enzyme library deposited in our laboratory.