Affinipure minimal cross reactivity secondary antibodies

Manufactured by Jackson ImmunoResearch

AffiniPure minimal cross reactivity secondary antibodies are designed to provide specific binding to target primary antibodies with minimal non-specific interactions. These secondary antibodies are produced to ensure high specificity and reduced background signal for accurate and reliable immunoassay results.

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Lab products found in correlation

C6219, by Merck Group (3 mentions) Ab11941, by Abcam (3 mentions) Ab5060, by Abcam (2 mentions) Alexa fluor 555 protein labeling kit, by Thermo Fisher Scientific (2 mentions) Hm1013, by Hycult Biotech (2 mentions) Af743, by R&D Systems (2 mentions) Ab19808, by Abcam (2 mentions) Alexa 647 streptavidin, by Jackson ImmunoResearch (2 mentions) Ab14055, by Abcam (2 mentions) Alexa 647, by Jackson ImmunoResearch (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Af3827, by R&D Systems (1 mentions) Lyve 1, by Thermo Fisher Scientific (1 mentions) A0082, by Agilent Technologies (1 mentions) L9393, by Merck Group (1 mentions) Sc 7294, by Santa Cruz Biotechnology (1 mentions)

3 protocols using affinipure minimal cross reactivity secondary antibodies

Immunostaining of Cell Junction Proteins

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Primary antibodies used for immunostaining were as follows: rabbit antibodies to Cx37 (Simon et al., 2006 (link)), Cx43 (C6219, Sigma), Prox1 (11–002, AngioBio; ab11941, Abcam), pHH3 (06–570, Millipore); rat antibodies to CD31 (550274, BD Biosciences); goat antibodies to Foxc2 (ab5060, Abcam), Vefgr3 (AF743, R&D Systems). AffiniPure minimal cross reactivity secondary antibodies (conjugated to Alexa 488, Cy3, Cy5, or Dylight 649) were from Jackson Immunoresearch.

Kanady J.D., Munger S.J., Witte M.H, & Simon A.M. (2015). Combining Foxc2 and Connexin37 deletions in mice leads to severe defects in lymphatic vascular growth and remodeling. Developmental biology, 405(1), 33-46.

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Immunostaining Antibody Protocols for Tissue Analysis

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Primary antibodies for immunostaining were: rabbit polyclonal antibodies to Collagen IV (ColIV) (ab19808, Abcam), Cx37 (18264) (Simon et al., 2006 (link)), Cx43 (C6219, Sigma), Cx47 (364700, Invitrogen), Laminin α5 (Lam α5) (405, a gift from Lydia Sorokin; used on whole mount samples) (Sixt et al., 2001 (link)), Prospero homeobox protein 1 (Prox1) (11-002, AngioBio), Prox1 (ab11941, Abcam), α-Smooth Muscle Cell Actin (SMA), Cy3 conjugated (C6198, Sigma); rat monoclonal antibody to Cluster of Differentiation 31 (CD31) (HM1013, Hycult Biotech), Laminin α5 (Lam α5) (4G6 A2 11, a gift from Lydia Sorokin; used on frozen sections) (Sixt et al., 2001 (link)), Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) (14-0443, eBioscience); goat polyclonal antibodies to Integrin α9 (Itga9), (AF3827, R&D Systems) Vascular Endothelial Growth Factor Receptor 3 (VEGFR3) (AF743, R&D Systems); chicken polyclonal antibodies to Laminin (Lam) (ab14055, Abcam). AffiniPure minimal cross reactivity secondary antibodies (conjugated to Alexa 488, Alex 555, Alexa 647, Cy3, Cy5, or Dylight 649) and Alexa 647 Streptavidin were from Jackson Immunoresearch or Invitrogen. Cx37 antibodies (18264) were directly labeled using the Alexa Fluor 555 Protein Labeling Kit (A20174, Invitrogen).

Munger S.J., Davis M.J, & Simon A.M. (2016). Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43. Developmental biology, 421(2), 204-218.

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Multicolor Immunostaining with Diverse Antibodies

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Primary antibodies for immunostaining were: rabbit polyclonal antibodies to collagen IV (ColIV) (ab19808, Abcam), Cx32 (71-0600, Invitrogen), Cx37 (18264) (Simon et al., 2006 (link)), Cx40 (Gabriels and Paul, 1998 (link)), Cx43 (C6219, Sigma), Cx43 (71-0700, Invitrogen), Cx47 (364700, Invitrogen), laminin α5 (Lam α5) (405, a gift from Lydia Sorokin) (Sixt et al., 2001 (link)), laminin (Lam) (L9393, Sigma), Prox1 (11-002, AngioBio), Prox1 (ab11941, Abcam), von Willebrand Factor, vWF (A0082, Dako); mouse monoclonal antibody to NFATc1 (sc-7294, Santa Cruz), smooth muscle cell actin (SMA), Cy3 conjugated; rat monoclonal antibody to CD31 (HM1013, Hycult Biotech), LYVE-1 (14-0443, eBioscience); goat polyclonal antibodies to EphB4 (AF446, R&D Systems), Foxc2 (ab5060, Abcam), integrin α9 (ITA9) (AF3827, R&D Systems), VEGFR3 (AF743, R&D Systems); chicken polyclonal antibodies to laminin (ab14055, Abcam); Syrian hamster monoclonal antibody to podoplanin (127402, Biolegend). AffiniPure minimal cross reactivity secondary antibodies (conjugated to Alexa 488, Alex 555, Alexa 647, Cy3, Cy5, or Dylight 649) and Alexa 647 Streptavidin were from Jackson Immunoresearch or Invitrogen. Cx37 antibodies (18264) were directly labeled using the Alexa Fluor 555 Protein Labeling Kit (A20174, Invitrogen).

Munger S.J., Geng X., Srinivasan R.S., Witte M.H., Paul D.L, & Simon A.M. (2016). Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice. Developmental biology, 412(2), 173-190.

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