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Fdglcu

Manufactured by Thermo Fisher Scientific
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FDGlcU is a fluorescent glucose analog product offered by Thermo Fisher Scientific. It serves as a tool for the study of glucose uptake and metabolism in various biological systems.

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Lab products found in correlation

Ampicillin, by GoldBio (1 mentions) Neomycin, by GoldBio (1 mentions) Vancomycin, by GoldBio (1 mentions) Click it plus tunel assay kit, by Thermo Fisher Scientific (1 mentions) Fitc dextran 70 kda, by Merck Group (1 mentions) Loperamide, by Merck Group (1 mentions)

3 protocols using fdglcu

1

In-Vivo GUS Activity Assay

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The GUS activity of the mice was detected using fluorescein di-β-D-glucuronide (FDGlcU, Thermo Fisher Scientific, Cat#F2915) by the method of Chen et al. as reported.38 (link) Mice (n = 6, per group) were administrated orally 7.3 μmol/kg FDGlcU dissolved in distilled deionized water. The fluorescence intensity in the intestine was detected on a Spectrum imaging system (Lago X, Spectral Instruments Imaging) 3 h after probe administration (λex: λem = 465 nm: 530 nm), with an exposure time was 5s. Fluorescence intensity was quantified by intestinal fluorescence using the Aura assay software.
Li P., Zhang R., Zhou J., Guo P., Liu Y, & Shi S. (2024). Vancomycin relieves tacrolimus-induced hyperglycemia by eliminating gut bacterial beta-glucuronidase enzyme activity. Gut Microbes, 16(1), 2310277.
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2

Fluorescent Imaging Probe Preparation

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FDGlcU was purchased from Thermo Fisher Scientific (Waltham, MA, U.S.A.) and reconstituted in DDW. The eβG inhibitor was purchased from Merck (Cat# 347423) (Darmstadt, Germany), reconstituted in DMSO at 4.255 mg/mL, and then further diluted with DMSO for in vitro assay or diluted with DDW to 0.2 mg/mL for in vivo imaging. Isoflurane was purchased from Aesica (Queenborough, U.K.). Fluorescein was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.), reconstituted in DMSO, and then diluted with DDW before use. Ampicillin, neomycin, and vancomycin were purchased from Gold Biotechnology (St. Louis, MO, U.S.A.).
Chen M., Cheng K.W., Chen Y.J., Wang C.H., Cheng T.C., Chang K.C., Kao A.P, & Chuang K.H. (2017). Real-time imaging of intestinal bacterial β-glucuronidase activity by hydrolysis of a fluorescent probe. Scientific Reports, 7, 3142.
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3

In Vitro Intestinal Permeability

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Morphine sulfate was obtained from National Institutes of Health [NIH]/National Institute on Drug Abuse [NIDA] (Bethesda, MD). Loperamide and 70 kDa FITC-Dextran were purchased from Sigma Aldrich (St. Louis, MO, USA). Click-iT® Plus TUNEL assay kit (Invitrogen™) and FDGlcU were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The β-Glucuronidase inhibitor 1-((6,8-Dimethyl-2-oxo-1,2-dihydroquinolin-3-yl)methyl)-3-(4-ethoxyphenyl)-1-(2-hydroxyethyl)thiourea (Calbiochem®), referred to as Inh-1 (Wallace et al., 2010 ; Saitta et al., 2014 (link)), was purchased from MilliporeSigma (Burlington, MA, USA). Irinotecan hydrochloride (CPT-11) was purchased from TCI America (Portland, OR, USA) and Thermo Fisher Scientific (Waltham, MA, USA). Clonidine hydrochloride was purchased from VWR (Radnor, Pennsylvania, USA).
Bachoual R., Dubreuil L., Soussy C.J, & Tankovic J. (2000). Roles of gyrA Mutations in Resistance of Clinical Isolates and In Vitro Mutants of Bacteroides fragilis to the New Fluoroquinolone Trovafloxacin. Antimicrobial Agents and Chemotherapy, 44(7), 1842-1845.
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