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2 protocols using dioxane

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Comprehensive Analytical Standards Sourcing

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The methanol, isopropanol, dioxane, and dichloromethane were purchased from VWR International (Radnor, Pennsylvania-US). The acetonitrile and ethanol were purchased from Carlo Erba, (Cornaredo-Milano, Italy). The aluminum chloride was purchased from Alfa Aesar (Haverhill, MA, USA). The natrium carbonate and 2,4-Dinitrophenylhydrazine (2,4-DNPH) were purchased from Fluka-Fisher scientific (Rodano-Milano, Italy). The potassium acetate and methanesulfonic acid were purchased from Acros Organics-Fisher scientific (Rodano-Milano, Italy). The thujone, daidzin, genistin, daidzein, genistein standards, and ethyl decanoate were purchased from Sigma-Aldrich-Merck (St. Louis, MO, USA).
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2

Fluorescent gelatin hydrogel synthesis

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Methacrylic anhydride, gelatin, fluorescein isothiocyanate isomer I (FITC), Irgacure 2959, collagenase type II, span 80, and HSA-MIR-218 (miR-218) were purchased from Sigma-Aldrich (St. Louis). Mineral oil, ethanol, dioxane, and borate buffer (pH = 9.5) were purchased from VWR Scientific (Seattle). Dialysis membranes (MWCO: 12–14 kDa) were obtained from Spectrum Lab (Dallas). PPP-RGI was synthesized as described by us previously.27 (link)
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