The cell cycle was analyzed by flow cytometry. After collecting cells post trypsinization, the cells were centrifuged and fixed overnight at 4 °C in 75% ice-cold ethanol. Cells were stained with 1 mL propidium iodide (PI) solution (20 μg/mL PI in the presence of RNase-A) for 30 min. Samples were analyzed on the BD FACSCanto™ II flow cytometer (BD Biosciences) and processed using the Cell Fit Cell Analysis program (Becton Dickinson Immunocytometry Systems, NJ, USA).
Cell fit cell analysis program
Manufactured by BD
Sourced in United States
The Cell Fit Cell Analysis program is a software tool designed for analyzing and quantifying cell-based assays. It provides automated cell detection, segmentation, and measurement capabilities to extract key parameters from cell samples. The program offers a range of analysis features to support various cell-based applications.
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Lab products found in correlation
2 protocols using cell fit cell analysis program
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis of Girinimbine in HT-29 Cells
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The cell cycle assay was done by flow cytometry. HT-29 cells were treated with girinimbine for 12, 24, and 48 hours in Rosewell Park Memorial Institute-1640 media with 10% fetal bovine serum. After collecting with trypsin–ethylenediaminet etraacetic acid solution (Sigma-Aldrich Co.), cells were fixed with 70% ethanol and incubated at 20°C for 30 minutes. Cells were then stained with 1 mL of PI staining solution (20 μg/mL PI in the presence of RNase-A) for 30 minutes on ice in the dark. Samples were analyzed using the BD FACSCanto™ II flow cytometer (BD Biosciences). The Cell Fit Cell analysis program (Becton Dickinson Immunocytometry Systems, NJ, USA) was used to analyze data from 10,000 cells per sample.
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