Sodium heparin anticoagulant collection tubes

Manufactured by BD

Sodium heparin anticoagulant collection tubes are used to collect blood samples for laboratory analysis. These tubes contain sodium heparin, an anticoagulant that prevents the blood from clotting, allowing for accurate testing of various blood components.

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Lab products found in correlation

Alsever s solution, by Merck Group (2 mentions) Mem medium, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lymphoprep density gradient medium, by STEMCELL (2 mentions) Rpmi 1640 medium, by GE Healthcare (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Allegra 6 centrifuge, by Beckman Coulter (1 mentions)

3 protocols using sodium heparin anticoagulant collection tubes

Isolation and Maintenance of Human RBCs and Bronchial Epithelial Cells

Cited 1 time

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Blood from consenting healthy human volunteers was collected in sodium heparin anticoagulant collection tubes (BD Biosciences; following the University of British Columbia and University of Otago Human Ethics guidelines). Red blood cells (RBCs) were isolated as described previously (Wolfmeier et al., 2018 (link)). In brief, phosphate buffered saline (PBS, Thermo Fisher/Gibco) diluted blood was layered into Lymphoprep density gradient medium (STEMCELL Technologies), centrifuged (500 × g for 20 min), and the RBCs present in the bottom layer of the density gradient were washed three times with PBS and finally resuspended in Alsever’s solution (Sigma-Aldrich) for storage at 4°C (maximum 4 weeks).
The human bronchial epithelial cell line 16HBE14o- (HBE, RRID:CVCL_0112) was kindly provided by Dr. D. Gruenert (University of California San Francisco). HBE cells were maintained in MEM medium (Thermo Fisher/Gibco) supplemented with 10% FBS, 2 mML-glutamine (Thermo Fisher/Gibco), and 1% penicillin/streptomycin (Thermo Fisher/Gibco) at 37°C in 5% CO₂ as described previously (Wolfmeier et al., 2018 (link)).

Wolfmeier H., Wardell S.J., Liu L.T., Falsafi R., Draeger A., Babiychuk E.B., Pletzer D, & Hancock R.E. (2022). Targeting the Pseudomonas aeruginosa Virulence Factor Phospholipase C With Engineered Liposomes. Frontiers in Microbiology, 13, 867449.

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Isolation and Culture of PBMCs, RBCs, and HBE Cells

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Peripheral blood mononuclear cells (PBMCs) and red blood cells (RBCs) were isolated from the blood of healthy, consenting human volunteers (following the University of British Columbia ethics guidelines). Blood was collected in sodium heparin anticoagulant collection tubes (BD Biosciences), diluted in phosphate buffered saline (PBS, ThermoFisher/Gibco) and layered onto Lymphoprep density gradient medium (STEMCELL Technologies). After centrifugation (500 ×g for 20 min) the buffy coat was transferred to a new tube, washed three times with PBS and resuspended in RPMI-1640 Medium (+25 mM HEPES, +l-Glutamine, GE Healthcare) supplemented with 10% fetal bovine serum (FBS, ThermoFisher/Gibco). PBMCs were seeded at density of 100,000 cells and rested.
RBCs were collected from the bottom of the density gradient, washed three times with PBS and stored for a maximum of 4 weeks in Alsever's solution (Sigma Aldrich).
The human bronchial epithelial cell line 16HBE14o- (HBE, RRID:CVCL_0112) was kindly provided by Dr. D. Gruenert (University of California San Francisco). HBE cells were maintained in MEM medium (ThermoFisher/Gibco) supplemented with 10% FBS, 2 mM l-glutamine (ThermoFisher/Gibco) and 1% penicillin/streptomycin (ThermoFisher/Gibco) at 37 °C in 5% CO₂. Cells were dissociated with 0.25% trypsin-EDTA (ThermoFisher/Gibco) at 80–90% confluency.

Wolfmeier H., Mansour S.C., Liu L.T., Pletzer D., Draeger A., Babiychuk E.B, & Hancock R.E. (2018). Liposomal Therapy Attenuates Dermonecrosis Induced by Community-Associated Methicillin-Resistant Staphylococcus aureus by Targeting α-Type Phenol-Soluble Modulins and α-Hemolysin. EBioMedicine, 33, 211-217.

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PBMC Isolation from Human Blood

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Peripheral Blood Mononuclear Cell (PBMC) Isolation Blood from healthy and consenting human volunteers (both male and female) was collected in sodium heparin anticoagulant collection tubes (BD Biosciences, Franklin Lakes, NJ), in accordance with the University of British Columbia ethics guidelines.
The blood was diluted in an equal volume of PBS buffer (pH 7.0) then layered onto Lymphoprep density gradient medium (STEMCELL Technologies Inc. Vancouver, BC) and centrifuged at $500 x g for 20 minutes in an Allegra 6 centrifuge (Beckman-Coulter, Brea, CA). The buffy coat was collected from the centrifuged samples, and PBMCs were washed three times with PBS and resuspended in 10% RPMI. Cell density was determined using Turks staining of a diluted sample of purified cells followed by counting using a hemacytometer.

Haney E.F., Wu B.C., Lee K., Hilchie A.L, & Hancock R.E.W. (2017). Aggregation and Its Influence on the Immunomodulatory Activity of Synthetic Innate Defense Regulator Peptides. Cell chemical biology, 24(8).

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