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Apilimod

Manufactured by Cayman Chemical
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Apilimod is a chemical compound that functions as a kinase inhibitor. It is primarily used in research applications for its ability to inhibit specific cellular processes. The core function of Apilimod is to modulate the activity of enzymes involved in signal transduction pathways.

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Lab products found in correlation

Fm4 64, by Thermo Fisher Scientific (3 mentions) Verapamil, by Cayman Chemical (2 mentions) Bafilomycin a1, by Cayman Chemical (2 mentions) Creatine kinase, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Quinacrine, by Merck Group (1 mentions) Cal520 dextran conjugate, by AAT Bioquest (1 mentions) Concanamycin a, by Cayman Chemical (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Merck Group (1 mentions) Calcofluor white solution, by Merck Group (1 mentions) Sb202190, by Cayman Chemical (1 mentions) Ly2228820, by Cayman Chemical (1 mentions) Ym201636, by Cayman Chemical (1 mentions) Skepinone l, by Cayman Chemical (1 mentions) Birb796, by Cayman Chemical (1 mentions) Vacuolin 1, by Cayman Chemical (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Acridine orange, by Merck Group (1 mentions)

3 protocols using apilimod

1

Soluble Reagents in Pipes-Sorbitol Buffer

Cited 1 time
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Soluble reagents were dissolved in Pipes-Sorbitol buffer (20 mM Pipes–KOH, pH 6.8, 200 mM sorbitol) with 125 mM KCl, unless indicated otherwise. C8-PI(3,5)P2 (1,2-dioctanoyl-phosphatidylinositol 3,5-bisphosphate) was purchased from Echelon Inc. ATP was purchased from RPI. Apilimod, bafilomycin A1, concanamycin A, and verapamil were from Cayman Chemical and dissolved in DMSO. AO, Calcofluor White solution, chloroquine, CoA, creatine kinase, EGTA, FCCP, and quinacrine were purchased from Sigma. Creatine phosphate was from Abcam. Cal-520 dextran conjugate molecular weight 10,000 was from AAT Bioquest. FM4-64 was purchased from ThermoFisher. Anti-Sec17 IgG (64 (link)), Pbi2 (Proteinase B inhibitor 2) (85 (link)), GST-ML1N (11 (link)), and GST-FYVE (38 (link)) were prepared as described and dialyzed against Pipes-Sorbitol buffer with 125 mM KCl.
Zhang C., Feng Y., Balutowski A., Miner G.E., Rivera-Kohr D.A., Hrabak M.R., Sullivan K.D., Guo A., Calderin J.D, & Fratti R.A. (2022). The interdependent transport of yeast vacuole Ca2+ and H+ and the role of phosphatidylinositol 3,5-bisphosphate. The Journal of Biological Chemistry, 298(12), 102672.
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2

Compound Preparation for Cell Culture

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Vacuolin-1 (#20425), YM201636 (#13576), Apilimod (#19094), SB202190 (#10010399), BIRB-796 (#10460), Skepinone-L (#16974), LY2228820 (#23259), Tak-715 (#26170) from Cayman Chemical, and WX8 and NDF from SPECS were dissolved in DMSO (Sigma Cat# D2650) at 20 mM stock concentration and diluted as necessary to be added to cell culture medium at 1:1,000 dilution. An equal volume of DMSO (vehicle) was added to control cells.
O’Connell C.E, & Vassilev A. (2021). Combined Inhibition of p38MAPK and PIKfyve Synergistically Disrupts Autophagy to Selectively Target Cancer Cells. Cancer research, 81(11), 2903-2917.
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3

Membrane Fusion Assay with Soluble Reagents

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Soluble reagents were dissolved in PIPES-Sorbitol (PS) buffer (20 mM PIPES-KOH, pH 6.8, 200 mM sorbitol) with 125 mM KCl unless indicated otherwise. Anti-Sec17 IgG (Mayer et al., 1996), and Pbi2 (Slusarewicz et al., 1997) were prepared as described previously. DiC8-PA (1,2-dioctanoyl-sn-glycero-3-phosphate) diC8-PI3P (1,2-dioctanoyl-phosphatidylinositol 3-phosphate), and diC8-PI(3,5)P2 (1,2-dioctanoylphosphatidylinositol 3,5-bisphosphate) were purchased from Echelon Inc. Apilimod, Verapamil, and Bafilomycin A1 were from Cayman Chemical and dissolved in DMSO. Acridine orange and FCCP (Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone were purchased from Sigma and dissolved in DMSO.
Miner G.E., Rivera‐Kohr D.A., Zhang C., Sullivan K.D., Guo A., & Fratti R.A. (2020). Reciprocal regulation of vacuolar calcium transport and V-ATPase activity, and the effects of Phosphatidylinositol 3,5-bisphosphate.
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