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Anti nanog

Manufactured by ReproCELL
Sourced in Japan

Anti-Nanog is a laboratory reagent used to detect and quantify the expression of the Nanog protein in cell samples. Nanog is a transcription factor that plays a crucial role in the maintenance of pluripotency and self-renewal in embryonic stem cells. Anti-Nanog can be used in various cell and molecular biology techniques, such as Western blotting, immunocytochemistry, and flow cytometry, to analyze Nanog expression levels in different cell types and experimental conditions.

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2 protocols using anti nanog

1

Stem Cell Marker Immunostaining

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For immunocytochemical staining, anti-Nanog (1:50; ReproCELL), anti-Oct3/4 h-134 (1:100; Santa Cruz Biotechnology), and anti-Sox-2 Y-17 (1:100; Santa Cruz) antibodies were used.
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2

Antibody Panel for Cell Analysis

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The primary antibodies used in this study were as follows: anti-phospho-H2A.X and anti-Sox2 (Millipore, Billerrica, MA, USA), anti-p53 and anti-PARP (Cell Signaling Technology, Beverly, MA), anti-p21, anti-Slug and anti-Twist (Santa Cruz Biotechnology, Dallas, TX), anti-p16 and anti-Cyclin B1 (BD Biosciences, San Diego, CA), anti-Cyclin D1 (MBL International, Nagoya, Japan), anti-Nanog (ReproCELL, Yokohama, Japan), anti-b-actin and anti-Vinculin (SIGMA-Aldrich, St. Louis, MO).
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