Human TNBC cell line MDA-MB-231 (American Type Culture Collection, ATCC) and MDA-MB-231-Luc (expressing luciferase reporter gene) were grown and cultured in DME/F-12 (1:1) medium (Thermo Scientific) containing 10% FBS inside a 37 °C incubator with 5% CO2 and a humidified atmosphere.
Dme f 12 1 1 medium
Manufactured by Thermo Fisher Scientific
DME/F-12 (1:1) medium is a cell culture medium formulation that provides a balanced combination of nutrients and components required for the growth and maintenance of a variety of cell types in vitro. It is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture. The medium supports the cultivation of various mammalian cell lines and is commonly used in biomedical research and cell culture applications.
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2 protocols using dme f 12 1 1 medium
1
Culturing TNBC Cell Lines
2
Serum-free Culture of Tumor Spheres
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Cells were seeded at a density of 3 × 104 cells and cultured in serum-free DME/F-12 (1:1) medium (ThermoFisher) supplemented with 10 ng/ml epidermal growth factor,10 ng/ml basic fibroblast growth factor and N2 (ThermoFisher) on ultralow attachment six-well plates (Corning, Corning, NY). Medium was replaced every three days. The spheres were cultured for 2 weeks, and then pictured and counted.
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