Sars cov 2 2019 ncov spike s1 d614g his recombinant protein

Fura-2-acetoxymethyl (AM) ester, capsaicin (CAP), iodo-resiniferatoxin (I-RTX, a specific TRPV1 blocker), AVE0991 (a non-peptide MASR1 agonist), Ang II, losartan (an AT1R blocker), dimethyl sulfoxide (DMSO), and amiloride were obtained from Sigma Aldrich. In addition, we used CALHM1 and CALHM3 Taqman primer assay mix (HS0736332_m1 and HS07290139_m1). SARS-CoV-2 (2019-nCoV) spike S1 (D614G)-His recombinant protein was obtained from Sino Biological. TRPV1 and ACE2 small interfering RNA (siRNA) (Qiagen FlexiTube Premix siRNA) were used to downregulate TRPV1 and ACE2, respectively. Scrambled siRNA (Qiagen) was used as a negative control. Pluronic F127 was obtained from Life Technologies.

Collagen-related peptide (CRP) was obtained from Prof. R. Farndale Laboratory (Cambridge, UK), and the collagen reagent Horm^® (equine) suspension was purchased from Takeda (St. Peter Strasse, Austria). Anti-CD62P conjugated FITC, anti-CD63 conjugated FITC, anti-PAC1 conjugated FITC, antibodies were obtained from BD Biosciences and the anti-GPVI antibody was obtained from Biocytex (Marseille, France). The SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His recombinant protein and SARS-CoV-2 BQ.1.1 (Omicron) Spike S1+S2 trimer proteins were sourced from SinoBiological (Beijing, China). All other reagents were purchased from Sigma Aldrich (Saint-Louis, USA). Supplementary Materials provide further details regarding the sources and references of the antibodies.