Thymopentin (TP5) was purchased from Meilunbio. Doxorubicin hydrochloride (DOX) was obtained from Aladdin. Fetal bovine serum albumin (FBS) was purchased from Biowest. Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco. All assay kits, if not specified, were acquired from Beyotime. The antibodies used were as follows: anti-calreticulin, HMGB1, and CD8 were purchased from Santa Cruz Biotechnology; Ki67 was purchased from Bioss; anti-mouse antibodies (FITC-labeled CD3, FITC-labeled CD11c, FITC-labeled CD25, PE-labeled CD8a, PE-labeled CD86, APC-labeled CD4, APC-labeled CD80, Alexa Flour 647-labeled Foxp3) were all purchased from BioLegend. D-Luciferin, Potassium Salt was purchased from Yeasen.
Fitc labeled cd11c
Manufactured by BioLegend
Sourced in United States
FITC-labeled CD11c is a fluorescently-conjugated antibody that specifically binds to the CD11c cell surface marker. CD11c is commonly expressed on dendritic cells and is involved in cell adhesion and migration processes.
Automatically generated - may contain errors
Lab products found in correlation
Pe labeled cd8a, by BioLegend (1 mentions)
Apc labeled cd4, by BioLegend (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
D luciferin potassium salt, by Yeasen (1 mentions)
Hmgb1, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum albumin fbs, by Biowest (1 mentions)
Anti calreticulin, by Santa Cruz Biotechnology (1 mentions)
Cellquest software, by BD (1 mentions)
Facscan flow cytometer, by BD (1 mentions)
2 protocols using fitc labeled cd11c
1
Comprehensive Immunomodulatory Assay Protocol
2
Analyzing Tolerogenic Dendritic Cells
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tolDCs were stained with FITC-labeled CD11c, MHC-II monoclonal antibody (mAb) and PE-conjugated CD80 or CD86 antibody (Biolegend, CA, USA), and they were then analyzed by flow cytometry using a FACScan flow cytometer and CellQuest software (Becton Dickinson, Mountain View, CA, USA). CD11-positive signals indicated the DC phenotype. MHC-II functions as the first stimulatory signal in the antigen-presenting process, while CD80 and CD86 are costimulatory molecules that promote T cell activation. The expression levels of CD80, CD86, and MHC-II should be decreased in tolDCs. The data are expressed as the mean fluorescence intensity (MFI).
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