Collagenase type II, Elastase, and Exisulind were obtained from Sigma-Aldrich co. (St Louis, MO, USA). PDGF-BB was purchased from R&D systems (Minneapolis, MN, USA). Goat anti-PKG I, rabbit anti-VE-cadherin, mouse anti-osteoponin, rabbit anti-CD31, and goat anti-actin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-VASP (Ser239), rabbit anti-t-Akt, and mouse anti-p-Akt (Ser473) were purchased from Cell Signaling (Berkely, MA, USA). Mouse anti-calponin antibody was obtained from Sigma-Aldrich co. (St Louis, MO, USA). Mouse anti-α-SMA antibody was purchased from Abcam (Cambridge, MA, USA). The secondary antibodies to each primary antibody were as follows. Anti-mouse IgG HRP conjugated and anti-rabbit IgG HRP conjugated antibodies were obtained from Promega (Madison, WI, USA). Anti-goat IgG HRP conjugated antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse IgG Alexa flour 488 and anti-rabbit IgG Alexa flour 594 secondary antibodies were purchased from Invitrogen (Carlsbad, CA, USA). Alzet osmotic pump was purchased from Durect corporation (Cupertino, CA, USA).
Anti rabbit igg alexa flour 594
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-rabbit IgG Alexa Fluor 594 is a secondary antibody conjugate that binds to rabbit primary antibodies. It is labeled with the Alexa Fluor 594 fluorescent dye, which has an excitation maximum of 590 nm and an emission maximum of 617 nm. This conjugate can be used for various immunodetection applications, such as Western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse igg alexa flour 488, by Thermo Fisher Scientific (3 mentions)
Lsm 700 confocal microscope, by Zeiss (2 mentions)
Elastase, by Merck Group (1 mentions)
Rabbit anti t akt, by Cell Signaling Technology (1 mentions)
Anti mouse α sma antibody, by Abcam (1 mentions)
Mouse anti p akt ser473, by Cell Signaling Technology (1 mentions)
Rabbit anti ve cadherin, by Santa Cruz Biotechnology (1 mentions)
Antigoat igg hrp conjugated antibody, by Santa Cruz Biotechnology (1 mentions)
Mouse anti calponin antibody, by Merck Group (1 mentions)
Rabbit anti cd31, by Santa Cruz Biotechnology (1 mentions)
Goat anti actin, by Santa Cruz Biotechnology (1 mentions)
Pdgf bb, by R&D Systems (1 mentions)
Collagenase type 2, by Merck Group (1 mentions)
Alzet osmotic pump, by Durect (1 mentions)
Ab133682, by Abcam (1 mentions)
Sc 126, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Prolong gold with dapi, by Thermo Fisher Scientific (1 mentions)
Tcs sp5 aobs confocal microscope, by Leica (1 mentions)
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
4 protocols using anti rabbit igg alexa flour 594
1
Vascular Smooth Muscle Cell Characterization
2
Immunofluorescence Staining of CTCF, p53, and ITGB4
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Cells grown on coverslips were fixed in 4% PFA. Antibodies against CTCF, p53 (sc-126; Santa Cruz,), ITGB4 (ab133682; Abcam) and the corresponding secondary antibodies (anti-rabbit IgG Alexa Flour 594 and anti-goat IgG Alexa Flour 488; Invitrogen) were used for detection. Cells were counter stained with DAPI (Invitrogen) and analyzed using a Zeiss LSM700 confocal microscope.
3
Immunofluorescence Assay for Transfected HEK293 Cells
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HEK293 cells were plated on to coverslips and transiently transfected using JetPrime transfection reagent (PolyPlus). Post transfection, cells were fixed using 3% paraformaldehyde for 30 min and permeabilized in 1x PBS, 0.1% Tween 20 (PBS-T), 0.1% Triton X-100 for 20 min at RT and further incubated in 5% goat serum diluted in PBS-T for 30 min at RT. Cells were then incubated with mouse anti-V5 (1:500; Thermo-Fisher) and/or rabbit anti-Flag tag (1:200; Thermo-Fisher; PA1-984B). The secondary antibodies anti-mouse IgG-Alexa Flour 488 (Thermo-Fisher; A11017) and/or anti-rabbit IgG-Alexa Flour 594 (Thermo-Fisher; A11037) were used at 1:200. Cells were washed in PBS-T overnight at 4 °C and coverslips embedded in ProLong Gold with DAPI (Thermo-Fisher) prior to imaging using TCS SP5 AOBS confocal microscope (Leica Microsystems).
4
Immunofluorescence Mapping of CD19 and IgG in the Brain
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Immunofluorescence staining of CD19 with specific cell-type antibodies was performed to determine the existence of CD19 expression in the brain. Brain sections was coincubated with CD19 (Abcam) antibody and NeuN (Abcam) antibody at 4 °C overnight. After that, sections were rinsed 3 times with 0.01 M PBS and incubated with anti-mouse IgG-Alexa Flour 488 (Thermo Scientific) and anti-rabbit IgG-Alexa Flour 594 at RT for 2 h in the dark. Then, incubated in the DAPI solution for 5 min in the dark, washed three times and mounted with anti-fluorescence quencher. Images were taken on a fluorescence microscope (Olympus Corporation, Tokyo, Japan).
Double immunofluorescence staining of IgG with specific cell-type antibodies was performed to determine the cellular localization of IgG in the brain. Brain sections was incubated with two antibodies: IgG (Thermo Scientific) and either Iba1 (Dako), NeuN (Abcam), or GFAP (Proteintech) and the following steps are the same as described above.
Double immunofluorescence staining of IgG with specific cell-type antibodies was performed to determine the cellular localization of IgG in the brain. Brain sections was incubated with two antibodies: IgG (Thermo Scientific) and either Iba1 (Dako), NeuN (Abcam), or GFAP (Proteintech) and the following steps are the same as described above.
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