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Sc 166394

Manufactured by Santa Cruz Biotechnology
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Sc-166394 is a laboratory consumable product manufactured by Santa Cruz Biotechnology. It is a multi-purpose tool designed for use in various scientific research applications. The core function of Sc-166394 is to facilitate sample preparation, handling, and analysis within the laboratory setting.

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Lab products found in correlation

Ac 40, by Merck Group (2 mentions) Sc 816, by Santa Cruz Biotechnology (2 mentions) Sc 67060, by Santa Cruz Biotechnology (2 mentions) Sc 20681, by Santa Cruz Biotechnology (2 mentions) Ma5 13065, by Thermo Fisher Scientific (2 mentions) Ma1 310, by Thermo Fisher Scientific (2 mentions) Sc 32233, by Santa Cruz Biotechnology (2 mentions)

2 protocols using sc 166394

1

Protein Expression and Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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2

Protein Expression and Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 µg of protein aliquots from cell lysates of testis from V7BAC transgenic mice were separated by 15% SDS-PAGE and transferred to nitrocellulose membranes. The membranes were probed with primary antibodies against VAMP7 (sc-166394, 1:100, Santa-Cruz or sc-67060, 1:50, Santa-Cruz), AR (sc-816, 1:1,000, Santa-Cruz), ESR1 (MA1-310, 1:200, Thermo-Fisher), LMNA (sc-20681, 1:200, Santa-Cruz), GAPDH (sc-32233, 1:200, Santa-Cruz), and ACTB (AC-40, 1:5,000, Sigma). HeLa cells were cotransfected with AR or ESR1 or VAMP7 or in combination. After 24 h of overexpression, cell lysates were prepared and immunoprecipitation was carried out using antibodies raised against AR (sc-816, Santa-Cruz) or ESR1 (MA5-13065, Thermo-Fisher) or VAMP7 (sc-67060, Santa-Cruz) or control IgG antibody. The samples were later subjected to western blotting with the indicated antibodies. Input was 5% of the lysates.
Tannour-Louet M., Han S., Louet J.F., Zhang B., Romero K., Addai J., Sahin A., Cheung S.W, & Lamb D.J. (2014). Increased gene copy number of the vesicle SNARE VAMP7 disrupts human male urogenital development through altered estrogen action. Nature medicine, 20(7), 715-724.
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