MDA-MB-231, A549, PANC-1, and B16F10 cells were cultured in dishes at a density of 1 × 105 cells/well for 24 h and divided into three groups: nanoparticles with smooth heads, nanoparticles with spiky heads, and nanorobots with spiky heads under 1 W cm−2 980 mm laser irradiation. Cells were treated with 100 μg/mL nanoparticles and incubated for 0 min, 5 min, 10 min, 15 min, 20 min and 30 min or combined with laser irradiation. Then, the supernatant was removed, and the cells were washed three times with cold PBS (HyClone, UT, USA). The cells were fixed with 1 mL of 4% formaldehyde (Beyotime Biotechnology, China) per well for 10 min at room temperature, and then washed three times with cold PBS. Finally, the membrane and nuclei were stained with 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt (DID, Beyotime Biotechnology, China) and 4,6-diamidino-2-phenylindole (DAPI, Beyotime Biotechnology, China) after permeabilization with 0.1% Triton X-100 (Beyotime Biotechnology, China), and images were taken by CLSM after DAPI staining.
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did
Manufactured by Beyotime
Sourced in China
1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt (DID) is a fluorescent dye used for labeling lipophilic structures. The compound contains a carbocyanine chromophore attached to two long alkyl chains.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Triton x 100, by Beyotime (1 mentions)
Formaldehyde, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Il 1β, by MedChemExpress (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate, by Beyotime (1 mentions)
Indocyanine green icg, by Maclin Biochemical Technology (1 mentions)
Tnf α, by MedChemExpress (1 mentions)
Western blot reagents, by Beyotime (1 mentions)
Interleukin 6 (il 6), by MedChemExpress (1 mentions)
3 3 dioctadecyloxacarbocyanine perchlorate, by Beyotime (1 mentions)
J774a 1 cell line, by Procell (1 mentions)
2 protocols using 1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did
1
Laser-Activated Nanoparticle Cytotoxicity Evaluation
2
Multimodal Imaging and Molecular Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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3,3′-dioctadecyloxacarbocyanine perchlorate
(DiO), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
(DiI), 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate
Salt(DiD), ROS flow kit, and Western blot reagents were purchased
from Beyotime Biotechnology (China). The cytokines used in this study
(TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)),
lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress
(MCE, China). Indocyanine green (ICG) was obtained from Macklin Inc.
(China). The apoptosis flow kit and cell counting kit-8 (CCK-8) cell
viability assay kit were purchased from Vazyme Biotech Co., Ltd. (China).
The J774A.1 cell line was purchased from Procell Life Science &
Technology Co., Ltd. (China). Lipofectamine 3000, PBS, media, serum,
and ELISA kits required for cell culture were purchased from Thermo
Fisher Scientific Inc. (Waltham, MA, USA). The PCR-related reagents
were purchased from Yeasen Biotechnology Co., Ltd. (China). The animals
needed for the experiment were purchased from the Laboratory Animal
Center of Huazhong University of Science and Technology.
(DiO), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate
(DiI), 1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine,4-Chlorobenzenesulfonate
Salt(DiD), ROS flow kit, and Western blot reagents were purchased
from Beyotime Biotechnology (China). The cytokines used in this study
(TNF-α, IL-1β, IL-6, interferon-γ (IFN-γ)),
lipopolysaccharide (LPS), and NGF were purchased from MedChemExpress
(MCE, China). Indocyanine green (ICG) was obtained from Macklin Inc.
(China). The apoptosis flow kit and cell counting kit-8 (CCK-8) cell
viability assay kit were purchased from Vazyme Biotech Co., Ltd. (China).
The J774A.1 cell line was purchased from Procell Life Science &
Technology Co., Ltd. (China). Lipofectamine 3000, PBS, media, serum,
and ELISA kits required for cell culture were purchased from Thermo
Fisher Scientific Inc. (Waltham, MA, USA). The PCR-related reagents
were purchased from Yeasen Biotechnology Co., Ltd. (China). The animals
needed for the experiment were purchased from the Laboratory Animal
Center of Huazhong University of Science and Technology.
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