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Pa5 29975

Manufactured by Thermo Fisher Scientific

The PA5-29975 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general laboratory use. The core function of this product is to perform a specific task within a laboratory setting. No further details on the intended use or performance characteristics of this product are provided.

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2 protocols using pa5 29975

1

Cellular Protein Quantification and Validation

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Cycloheximide (01810) and calf-intestinal alkaline phosphatase (CIP, P4978) were purchased from Sigma. Dimethyl sulfoxide (D8418, Sigma-Aldrich, St Louis, MO, USA) was added to the cultures at 0.1% (v/v) as a solvent control. Specific antibodies against ATP5C1 (PA5-29975) and NDUFA9 (459100) were purchased from Invitrogen. Antibodies specific for ATP5A1 (51), SIRT3 (14.45), VDAC (B-6), SDHA (B-1), UQCRC2 (G-10), COX IV (D-20), PGC-1α (H-300), PGC-1β (E-9), NRF1 (H-285), NRF2 (C-20), Tfam (H-203), SIRT1 (H-300), PKCα (C-20), actin (I-19), V5 (H-9), Flag (D-8), His (AD1.1.10) and GST (B-14) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
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2

Protein Expression Regulation Analysis

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The target shRNA plasmid DNA human PKCα (RHS4531-EG5578) and SIRT3 (RHS4531-EG23410) were purchased from Open Biosystems. Specific antibodies against ATP5C1 (PA5-29975) was purchased from Invitrogen. Abs specific for ATP5A1 (51), SIRT3 (14.45), VDAC (B-6), SDHA (B-1), UQCRC2 (G-10), COX IV (D-20), PGC-1β (E-9), Tfam (H-203), PKCα (C-20), Actin (I-19), V5 (H-9), Flag (D-8), His (AD1.1.10), Lys-AC (AKL5C1), and GST (B-14) were purchased from Santa Cruz Biotechnology.
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