Hp 6890n plus chromatograph

Gas chromatography (GC) analysis was performed on an Agilent Technologies HP 6890 Plus Gas chromatograph equipped with a FID and fitted with HP-5MS column (30 m x 0.25 mm, film thickness 0.25 m, Agilent Technology). The analytical conditions were: carrier gas H2 (1 mL/min), injector temperature (PTV) 250ºC, detector temperature 260ºC, column temperature programmed from 60ºC (2 min hold) to 220ºC (10 min hold) at 4 o C/min. Samples were injected by splitting and the split ratio was 10:1. The volume injected was 1.0 L. Inlet pressure was 6.1 kPa. Each analysis was performed in triplicate. The relative amounts of individual components were calculated based on the GC peak area (FID response).
An Agilent Technologies HP 6890N Plus Chromatograph fitted with a fused silica capillary HP-5 MS column (30 m x 0.25 mm, film thickness 0.25 m) and interfaced with a mass spectrometer HP 5973 MSD was used for the GC/MS analysis, under the same conditions as those used for GC analysis. The conditions were the same as described above with He (1 mL/min) as a carrier gas. The MS conditions were as follows: ionization voltage 70 eV; emission current 40 mA; acquisitions scan mass range of 35-350 amu at a sampling rate of 1.0 scan/s. The MS fragmentation patterns were checked with those of other essential oils of known composition.