After bisulfite conversion, we determined DNA methylation patterns in paired tumor and normal tissues collected from five Taiwanese patients using Infinium Human Methylation 450K BeadChips (Illumina, San Diego, CA, USA) for one patient and EPIC Methylation Beadchips (Illumina) for four patients. In the DNA methylation assay, beta values were used to score the methylation level. Beta values are the ratio of the intensity of the methylated signal to the overall (sum of methylated and unmethylated) signal and thus ranged from 0 (no methylation) to 1 (full methylation). To design TSTD1 methylation–specific primers, we used MethPrimer (42 (link)) and Methyl Primer Express (v1.0, Thermo Fisher Scientific).
Epic methylation beadchips
Manufactured by Illumina
Sourced in United States
The EPIC Methylation Beadchips are an array-based platform designed for genome-wide DNA methylation analysis. The beadchips provide comprehensive coverage of the human methylome, enabling the examination of over 850,000 methylation sites across the genome.
Automatically generated - may contain errors
2 protocols using epic methylation beadchips
1
DNA Methylation Profiling in Cancer
2
Epigenetic Profiling of Tumor DNA
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A total of 250 ng of tumor-derived DNA was bisulfite-converted and hybridized to Illumina Epic methylation bead chips. Epigenetic classification was performed as published by Capper et al. 27
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