γ irradiated m tb h37rv

Manufactured by BEI Resources

γ-irradiated M. tb H37Rv is a laboratory product containing γ-irradiated Mycobacterium tuberculosis strain H37Rv. This product is a source of the inactivated M. tb strain for research purposes.

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Lab products found in correlation

Anti ifn γ, by BD (1 mentions) Cyclosporin a, by Merck Group (1 mentions) Anti cd4 fitc, by Thermo Fisher Scientific (1 mentions) Anti il 22, by Thermo Fisher Scientific (1 mentions) Dcfda cellular reactive oxygen species detection assay kit, by Abcam (1 mentions) Pecy5 anti foxp3, by Thermo Fisher Scientific (1 mentions) Anti cd25 apc, by Thermo Fisher Scientific (1 mentions) Anti il 10 pe, by Thermo Fisher Scientific (1 mentions) Anti foxp3 pe, by Thermo Fisher Scientific (1 mentions) Anti cd3 clone ucht1, by Thermo Fisher Scientific (1 mentions) Fitc anti cd8 clone 53 6, by BioLegend (1 mentions) Ag85a, by BEI Resources (1 mentions)

Close spelling variants of this product

H37Rv (2 citations)

2 protocols using γ irradiated m tb h37rv

Comprehensive Flow Cytometry Protocol for Immune Cell Analysis

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For flow cytometry, we used FITC anti-CD4, allophycocyanin (APC) anti-CD4, APC anti-CD25, PE anti-Foxp3, PE-Cy5 anti-Foxp3 (all from eBioscience), FITC anti-CD14, FITC anti-CD8, PE anti-PD1, FITC anti-D4GDI (Pierce), PE anti-IL-10, PE anti-TGF-β and PE anti-IFN-γ and PE anti-CD127 (e-Bioscience), APO-Direct TUNEL kit (e-Bioscience), Caspase-3 staining kit (BD Pharmingen) and DCFDA—Cellular Reactive Oxygen Species Detection Assay Kit (Abcam). γ-irradiated M. tb H37Rv was obtained from BEI Resources. In some experiments recombinant D4GDI (Cell Sciences) and cyclosporin A (Sigma) were used. For neutralization experiments, anti-IFN-γ (BD Pharmingen) and anti IL-22 (e-Biosciences) antibodies were used.

Venkatasubramanian S., Dhiman R., Paidipally P., Cheekatla S.S., Tripathi D., Welch E., Tvinnereim A.R., Jones B., Theodorescu D., Barnes P.F, & Vankayalapati R. (2015). A Rho GDP Dissociation Inhibitor Produced by Apoptotic T-Cells Inhibits Growth of Mycobacterium tuberculosis. PLoS Pathogens, 11(2), e1004617.

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Comprehensive Immune Cell Profiling

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FITC anti-CD4 (clone GK1.5, Biolegend), FITC anti-CD8 (Clone 53–6.7, Biolegend), FITC anti-CD14 (Clone 61D3, eBiosciences), qdot605 anti-CD14 (clone Tük4, Invitrogen), V450 anti-CD2 (clone RPA-2.10, eBioscience), anti-CD3 (clone UCHT1, eBioscience), anti-CD19 (clone HIB19, eBioscience), anti-CD20 (clone 2H7, eBioscience), anti-CD56 (clone B159, eBioscience), PE-Cy7 anti-CD16 (clone 3G8, Biolegend), APC-Cy7 anti-HLA-DR (clone L243, BD biosciences), PE anti-CD142/TF (clone HTF-1,eBioscience) and PerCP-Cy5.5 anti-TNF-α (clone MAb11, eBioscience) were used for flow cytometry, γ-irradiated M. tb H37Rv and Ag85a was obtained from BEI Resources.

Venkatasubramanian S., Tripathi D., Tucker T., Paidipally P., Cheekatla S., Welch E., Raghunath A., Jeffers A., Tvinnereim A.R., Schechter M.E., Andrade B.B., Mackman N., Idell S, & Vankayalapati R. (2015). TISSUE FACTOR EXPRESSION BY MYELOID CELLS CONTRIBUTES TO PROTECTIVE IMMUNE RESPONSE AGAINST Mycobacterium tuberculosis INFECTION. European journal of immunology, 46(2), 464-479.

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