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Ika c mag hs 7

Manufactured by IKA Group
Sourced in Germany

The IKA® C-MAG HS 7 is a magnetic stirrer designed for laboratory use. It provides a stirring function for mixing liquids in a controlled environment.

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5 protocols using ika c mag hs 7

1

Topical Emulgel and Oleogel Formulations

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All the experimental formulations compositions are shown in Table 1. Gel was prepared using poloxamer 407. The appropriate content of the poloxamer was weighed (18.0% (w/v)) and mixed with the appropriate content of purified water, and the mixtures were left in a refrigerator (5 °C) for 24 h until dissolved and homogeneous gel forms were obtained. A total of 10.0 g of glycerin, 10.0 g of olive oil, 6.0 g of Span 20, 6.0 g of Tween 20, and water ad 100.0 ± 0.5 g were mixed with a magnetic stirrer IKA® C-MAG HS 7 (IKA®-Werke GmbH & Co. KG, Staufen Im Breisgau, Germany) until homogeneous emulsion forms were obtained. Equal amounts of gel (10.0 g) and emulsion (10.0 g) were mixed with a magnetic stirrer until an emulgel of homogeneous structure was obtained. Oleogel was modeled using PIONIER® PLW basis. Vaseline was the basis for the ointment. All the experimental formulations were stored in a refrigerator (at 5 °C).
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2

Purification of Raw Lactide by Recrystallization

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The purification of raw lactide from residual oligomers, lactic acid and water was carried out by recrystallization from ethanol, isopropanol, ethyl acetate and butyl acetate. The process was carried out in a 100 mL beaker on a heating plate equipped with a magnetic stirrer. The ratio of raw lactide:solvent in the case of alcohols and ethyl acetate was taken in the mass ratio of 2:1, and in case of butyl acetate—3:1. The temperature of 70 °C and stirring were maintained by a heating plate, IKA C-MAG HS-7 (IKA-Werke GmbH & Co., KG, Staufen, Germany). After complete dissolution of the substance, the beaker was removed from the hotplate and cooled to 5 °C. Then, lactide crystals were separated from the solvent by using Buchner funnel vacuum filtration.
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3

Carbomer-Based Antioxidant Emulgel Formulations

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Gels were formulated using different concentrations of carbomer. The appropriate amount of carbomer was weighed and mixed with the proper content of purified water; the mixtures were kept at room temperature for 24 h. Next, 10% (v/v) sodium hydroxide was added dropwise to the formulations until the pH value reached 6.2 and homogeneous gel forms were obtained. Glycerin, castor oil, Span 20, Tween 20, and an appropriate amount of water were mixed with a magnetic stirrer IKA® C-MAG HS 7 (IKA®-Werke GmbH & Co. KG, Staufen Im Breisgau, Germany) until homogeneous emulsions were prepared. Equal contents of gels (10 g) and emulsions (10 g) were mixed with a magnetic stirrer until emulgels of homogeneous structure were formed. We chose to add 1% aqueous dry extracts of phenolic compounds to each prepared gel and emulgel formulation. All the experimental formulations were stored in a refrigerator (at 5 °C) (Table 1).
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4

Preparation and Characterization of CLX-DES Solution

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The final CLX–DES system containing 8 mg CLX/g DES was prepared by dissolving CLX in DES at a 900-rpm stirring rate at an ambient temperature with a magnetic stirrer (IKA C-MAG HS7, Ika-Works GmBH&Co., Staufenim Breisgau, Germany). A transparent, clear, and viscous solution was generated and stored in a desiccator. The prepared CLX–DES formulation was further characterized by PLM and FTIR using the method described in Section 2.2.1.
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5

Zein-Caseinate-Pectin Particle Synthesis

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Caseinate and pectin aqueous solutions were separately prepared and then adjusted to pH 3.5 using HCl. The caseinate aqueous solution (1 mg/mL) was added slowly to the same volume of the pectin aqueous solution with concentrations ranging from 0.2 to 2 mg/mL under continuous stirring using a magnetic stirrer (IKA®C-MAG HS 7, IKA WORKS Inc., Wilmington, NC, USA) at ambient temperature. The pectin to caseinate mass ratio used throughout this study was from 0.2:1 to 2:1 (w/w).
Zein was dissolved in 80 mL/100 mL aqueous ethanol solutions to obtain a stock solution with the final concentration of 10 mg/mL. For preparation of zein-caseinate-pectin particles, a liquid-liquid dispersion method was used following a laboratory procedure [11 (link)] with some slight modifications. Briefly, 1 mL of zein stock solution was added slowly into 10 mL of the diluted caseinate-pectin complex dispersions (pH 3.5) with continuous stirring for 30 min at ambient temperature. Then, N2 was flowed to remove the ethanol from the system using a nitrogen evaporator (N-EVAPTM 112, Organomation Associates Inc., Berlin, MA, USA). The final dispersions were centrifuged at 4500 rpm for 5 min to remove any large particles and then adjusted to a series of acidic pH values by adding HCl or NaOH.
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