Reagents used in this study included genomic DNA extraction kit (Omega Bio-Tek, Norcross, CA, USA), SYBR green fluorescent dye (Quanshijin Technology Co., Ltd. Beijing, China) and primers, and plasmids (Shenggong Bioengineering Co., Ltd. Shanghai, China). Instrument used in the study included an electronic balance (Precision Scientific Instrument Co., Ltd. Shanghai, China), a Heal Force ultrapure water system (Likang Biomedical Technology Holding Group, Hong Kong, China), and a real-time fluorescent quantitative PCR machine (Bio-Rad CFX96, Hercules, CA, USA).
Genomic dna extraction kit
Manufactured by Omega Bio-Tek
Sourced in United States
The Genomic DNA Extraction Kit is a laboratory product designed to efficiently extract and purify high-quality genomic DNA from a variety of sample types. The kit utilizes a rapid and reliable protocol to isolate DNA, making it a useful tool for various applications in molecular biology, genetics, and genomic research.
Automatically generated - may contain errors
Lab products found in correlation
Cfx96, by Bio-Rad (1 mentions)
Polyethylene glycol peg 20 000, by Solarbio (1 mentions)
Ribo off rrna depletion kit, by Vazyme (1 mentions)
Plasmid extraction kit, by Omega Bio-Tek (1 mentions)
Clonexpress 2 one step cloning kit, by Vazyme (1 mentions)
Mito tracker green kit, by Beyotime (1 mentions)
Chamq sybr qpcr master mix kit, by Vazyme (1 mentions)
Nrf2 inhibitor ml385, by MedChemExpress (1 mentions)
Fitc dextran, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Ros detection kit, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
Mmp assay kit, by Beyotime (1 mentions)
Rnase r, by Beyotime (1 mentions)
Mirna extraction kit, by Sangon (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Bulge loop mirna qrt pcr starter kit, by RiboBio (1 mentions)
5 protocols using genomic dna extraction kit
1
DNA Extraction and Real-Time PCR Analysis
2
SNP Genotyping for GC Association
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To evaluate the association between the three SNPs and GC, peripheral venous blood samples (5 mL) were collected from all subjects in EDTA vacutainers. Genomic DNA was obtained from the peripheral blood lymphocytes of study subjects using the Genomic DNA Extraction Kit (Omega Bio-Tek, Norcross, GA, USA, or GoldMag Ltd., Xi'an, China) according to the manufacturers' protocol. All samples were collected before curative resection and stored at −80°C for subsequent analysis. The GJA1 gene rs2071165 G > A, SCAMP1 gene rs4530741 A > C, and SCAMP1 gene rs6874309 T > A polymorphisms were genotyped on the Agena MassARRAY RS1000 platform according to the standard protocol (Applied Biosystems, Foster City, CA, USA). Primers were designed using the Agena MassARRAY Assay Design 4.0 software.
3
Characterization of L. fermentum RC4 Strain
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L. fermentum RC4 was preserved at the China General Microbiological Culture Collection Center (CGMCC NO. 8212). De Man, Rogosa and Sharpe (MRS) agar and broth medium were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Shandong, China), and the Luria-Bertani (LB) medium was purchased from Fuyuan Biotch Co., Ltd. (Shanghai, China). The pET-28a-EGFP plasmid was purchased from Fenghui Biotech Co. Ltd (Hunan, China), and the Stbl3 E. coli competent cells were purchased from TransGen Biotech Co., Ltd. (Beijing, China). Polyethylene glycol (PEG) 20000 was purchased from Solarbio Technology Co., Ltd. (Beijing, China). The genomic DNA extraction kit, plasmid extraction kit, gel recycling kit, and reverse transcription PCR kit were obtained from Omega Bio-tek, Ink. (Georgia, USA). TemplatePrepKit 1.0 kit was obtained from KAPA Biosystems (Massachusetts, USA). ClonExpress II One Step Cloning Kit, and Ribo-off rRNA Depletion kit were obtained from Nanjing Vazyme Biotech Co., Ltd. (Jiangsu, China). HiPure Bacterial RNA Kit was obtained from Magen Biotech Co., Ltd. (Shanghai, China). The nitrite reductase activity assay kit was purchased from Suzhou Comin Biotech Co., Ltd. (Jiangsu, China). The nitrite detection kit was purchased from Nanjing Jiancheng Technology Co., Ltd. (Jiangsu, China). Other reagents are analytical grade.
4
Nrf2-mediated Antioxidant Response Assay
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Sodium selenite, fluorescein isothiocyanate-dextran (FITC-dextran) with average molecular weight 4,000 and H2O2 were purchased from Sigma-Aldrich Co.. Nrf2 inhibitor (ML385) was purchased from MedChemExpress (Shanghai, People's Republic of China). All reagents for cell culture were purchased from Life Technologies (Thermo Fisher Scientific, Waltham, MA, USA). Mito-Tracker Green Kit, ATP Assay Kit, MMP Assay Kit, ROS detection Kit, and Cell Counting Kit-8 (CCK-8) were purchased from Beyotime Biotechnology (Shanghai, People's Republic of China). RIPA lysis buffer and bicinchoninic acid (BCA) protein assay kit were purchased from Solarbio Life Sciences Biotech Co. (Beijing, People's Republic of China). Nrf2, NQO-1, and HO-1 primary antibodies and corresponding secondary antibody were purchased from Abcam Biotechnology (Cambridge, MA, USA). Genomic DNA extraction Kit was purchased from OMEGA Bio-Tek (Norcross, GA, USA). ChamQTM SYBR® qPCR Master Mix Kit was purchased from Vazyme (NanJing, People's Republic of China).
5
Profiling circRNA, mRNA, and miRNA expression
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Total RNA was extracted by a RNeasy® Mini Kit (QIAGEN, Hilden, Germany). RNase R (Beyotime, Shanghai, China) was used to detect circRNA. Genomic DNA (gDNA) was isolated using a Genomic DNA Extraction Kit (Omega Bio-Tek, Norcross, GA, USA). Quantification of RNA and gDNA was performed using the TOROIVD® qRT Master Mix and SYBR Green qPCR Master Mix (TOROIVD, Shanghai, China). miRNA was isolated using the miRNA Extraction Kit (Sangon Biotech, Shanghai, China), and a Bulge-Loop miRNA qRT-PCR Starter Kit (RiboBio, Guangzhou, China) was used to perform real-time PCR. The circRNA and mRNA levels were normalised to those of β-actin, and miRNA was normalised to that of U6 and determined using the 2−ΔΔCt method. The primer sequences used in this study are shown in Additional file 3 : Table S2.
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