Dimethyldioctadecylammonium dda

1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC); L-a-phosphatidylcholine hydrogenated (Soy) (HSPC); 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC); 1,2-dioleoyl-3-dimethylammonium-propane (DAP); dimethyldioctadecylammonium (DDA); and D-(+)-trehalose 6,6’-dibehenate (TDB) were purchased from Avanti Polar Lipids Inc., (Albaster, AL, USA). Chloroform and methanol were purchased from LabScan (Dublin, Ireland). The poly(oligoethylene glycol acrylate)-b-poly(lauryl acrylate) (POEGA-PLA) (Figure 1a) block copolymer was synthesized using RAFT polymerization methodologies. The POEGA block was prepared first and then used as a macromolecular chain transfer agent for the synthesis of the second PLA block. The poly(2-(dimethylamino)ethyl methacrylate)-b-poly(lauryl methacrylate) (PDMAEMA-b-PLMA) (Figure 1b) amphiphilic diblock copolymer was synthesized using RAFT polymerization methodologies [30 (link)].

Dimethyldioctadecylammonium (DDA) (Figure 1a) and D-(+)-trehalose 6,6′-dibehenate (TDB) (Figure 1b) were purchased from Avanti Polar Lipids Inc. (Albaster, AL, USA); chloroform and methanol was purchased from LabScan (Dublin, Ireland). The PDMAEMA-b-PLMA (Figure 1c) amphiphilic diblock copolymer was synthesized with RAFT polymerization methodologies [23 (link)]. PAMAM dendrimer generation 4 (PAMAM G4) (Figure 1d) (empirical formula C₆₂₂H₁₂₄₈N₂₅₀O₁₂₄, formula weight: 14,214.17) was purchased from Aldrich. Bovine serum albumin was purchased from Merck (Darmstadt, Germany) and Pierce™ (Pittsburgh, PA, USA). The BCA protein assay kit was purchased from Thermo Scientific™, Waltham, MA, USA.