Plc prf 5

Manufactured by Keygen Biotech

Sourced in China

The PLC/PRF/5 is a laboratory equipment designed for cell culture applications. It functions as a cell culture incubator, providing a controlled environment for the growth and maintenance of cells. The core purpose of the PLC/PRF/5 is to maintain the optimal temperature, humidity, and atmospheric conditions required for cell culture experiments.

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Lab products found in correlation

7 protocols using plc prf 5

Cell Culture of Hepatocellular Carcinoma

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The HCC cell lines (HepG2 and PLC/PRF/5) were purchased from KeyGen Biotech (Nanjing, China) and cultured in Dulbecco's modified Eagle's medium and RPMI 1640 medium, respectively. The complete medium was supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin. The cells were maintained at 37°C in a humidified atmosphere including 5% CO₂.

Cai S., Bi Z., Bai Y., Zhang H., Zhai D., Xiao C., Tang Y., Yang L., Zhang X., Li K., Yang R., Liu Y., Chen S., Sun T., Liu H, & Yang C. (2020). Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1. Frontiers in Oncology, 9, 1431.

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Human Liver Cancer Cell Maintenance

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The HepG2, PLC/PRF/5 and SMMC-7721 human liver cancer cell line was from KeyGen Biotech. All cells were passaged and maintained in RPMI 1640 containing 10% fetal bovine serum (Hyclone, USA) and maintained at 37°C with 5% CO₂ in a humidified atmosphere.

Qin Y., Yang G., Li M., Liu H.J., Zhong W.L., Yan X.Q., Qiao K.L., Yang J.H., Zhai D.H., Yang W., Chen S., Zhou H.G., Sun T, & Yang C. (2017). Dihydroartemisinin inhibits EMT induced by platinum-based drugs via Akt–Snail pathway. Oncotarget, 8(61), 103815-103827.

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Cell Culture Protocols for Cancer Research

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The human carcinoma cell lines A549, HEK-293, PLC-PRF-5, H446, LLC, MCF-7, MDA-MB-231, HCT-116 and BL16F10 were obtained from KeyGen Biotech (Nanjing, China). The cells were cultured in RPMI-1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) and antibiotics (50 units/mL penicillin and 50 μg/mL streptomycin). All cultures were maintained at 37°C in a humidified atmosphere containing 5% CO₂.

Zhong W., Chen S., Zhang Q., Xiao T., Qin Y., Gu J., Sun B., Liu Y., Jing X., Hu X., Zhang P., Zhou H., Sun T, & Yang C. (2017). Doxycycline directly targets PAR1 to suppress tumor progression. Oncotarget, 8(10), 16829-16842.

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Hypoxic Liver Cancer Cell Lines

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Human liver cancer cell lines, namely, PLC/PRF/5, SMMC-7721, and HepG2, were purchased from KeyGen Biotech (Nanjing, China). All the cell lines were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum (HyClone, USA) and 1% penicillin–streptomycin in a humidified atmosphere (37 °C, 5% CO₂). To obtain a hypoxic condition, the cells were cultured in a CO₂ incubator with 94% N₂, 5% CO₂, and 1% O₂. Cells were put in hypoxic conditions for 24 h concurrently treated with salidroside (the time of wound-healing assay is 48 h).

Qin Y., Liu H.J., Li M., Zhai D.H., Tang Y.H., Yang L., Qiao K.L., Yang J.H., Zhong W.L., Zhang Q., Liu Y.R., Yang G., Sun T, & Yang C. (2018). Salidroside improves the hypoxic tumor microenvironment and reverses the drug resistance of platinum drugs via HIF-1α signaling pathway. EBioMedicine, 38, 25-36.

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Culturing Human Cancer Cell Lines

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Human carcinoma cell lines MDA-MB-231, NIH-3T3 and PLC-PRF-5 were obtained from KeyGen Biotech (Nanjing, China). The cells were grown in RPMI-1640 medium (Hyclone; GE Healthcare, Connecticut, US) and were supplemented with 10% fetal bovine serum (FBS) (Hyclone) and antibiotics (50 units/mL penicillin and 50 μg/mL streptomycin). All cultures were maintained at 37 °C in a humidified atmosphere containing 5% CO₂.

Zhong W., Sun B., Lu C., Yu H., Wang C., He L., Gu J., Chen S., Liu Y., Jing X., Bi Z., Yang G., Zhou H., Sun T, & Yang C. (2016). Problems and Solutions in Click Chemistry Applied to Drug Probes. Scientific Reports, 6, 35579.

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Cell Line Transfection with STAT3 siRNA

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The following human cell lines were obtained from KeyGen Biotech (Nanjing, China): liver (PLC/PRF/5), lung (NCI-A549), breast (MCF-7), and colon (HCT-8) cancer cell lines. The cells were grown in 1640 medium supplemented with 10% fetal bovine serum (Hyclone, USA) and maintained at 37 °C with 5% CO₂ in a humidified atmosphere. The liver (HepG2) and pancreatic (PANC-1) cancer cell lines were cultured in DMEM medium supplemented with 10% fetal bovine serum (Hyclone, USA) and maintained at 37 °C with 5% CO₂ in a humidified atmosphere. The siRNA of STAT3 was obtained from GenePharma Technology (Shanghai, China). The siRNAs of STAT3 and siRNA control vector were transfected into PLC/PRF/5 and HepG2 cells with Lipofectamine 2000 (Invitrogen, USA).

Yang L., Zhang X.Y., Li K., Li A.P., Yang W.D., Yang R., Wang P., Zhao Z.H., Cui F., Qin Y., Yang J.H., Tao H.L., Sun T., Chen S., Yu P.H., Liu H.J, & Yang C. (2019). Protopanaxadiol inhibits epithelial–mesenchymal transition of hepatocellular carcinoma by targeting STAT3 pathway. Cell Death & Disease, 10(9), 630.

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Cell Culture Maintenance Protocol

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Hela, PLC-PRF-5, HepG2, MHCC-97L, MHCC-97H, LO2, THLE2, Hep3B, and SK-Hep-1 cells were purchased from KeyGEN BioTECH and OBIO, Shanghai. Cells are maintained in RPMI 1640 or DMEM medium (Neuronbc) with 10%FBS and 1%PS. All the cell lines were kept at 37 °C under a humidified atmosphere of 5% CO₂ in an incubator, trypsinized, and passaged every 2 days. Plasmid DNA was transfected using Lipofectamine 2000 transfection reagent (Invitrogen).

Meng J., Ai X., Lei Y., Zhong W., Qian B., Qiao K., Wang X., Zhou B., Wang H., Huai L., Zhang X., Han J., Xue Y., Liang Y., Zhou H., Chen S., Sun T, & Yang C. (2019). USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma. Theranostics, 9(2), 573-587.

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