Bcpap
Manufactured by American Type Culture Collection
Sourced in United States, China
The BCPAP is a laboratory instrument designed for the quantification of bacterial and cell growth. It provides accurate and precise measurements of optical density, a common method to determine cell population in a liquid culture. The BCPAP is a compact, easy-to-use device that supports multiple sample analysis simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Tpc 1, by American Type Culture Collection (52 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (34 mentions)
Nthy ori 3 1, by American Type Culture Collection (26 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (16 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (14 mentions)
Bcpap, by Thermo Fisher Scientific (12 mentions)
Tpc 1, by Thermo Fisher Scientific (11 mentions)
Ktc 1, by American Type Culture Collection (9 mentions)
Nthy ori 3 1, by Thermo Fisher Scientific (8 mentions)
Penicillin, by Thermo Fisher Scientific (7 mentions)
Ht ori3, by American Type Culture Collection (7 mentions)
Rpmi 1640, by Thermo Fisher Scientific (6 mentions)
Ihh 4, by American Type Culture Collection (6 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Cal 62, by American Type Culture Collection (4 mentions)
Hth83, by American Type Culture Collection (4 mentions)
Sw579, by American Type Culture Collection (3 mentions)
Dmem medium, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Merck Group (3 mentions)
71 protocols using bcpap
1
PTC Cell Lines Cultivation
2
Establishment and Maintenance of Cell Lines
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FreeStyle™ 293T cells were obtained from Thermo Fisher Scientific (Gibco, Carlsbad, CA, USA) and cultured in FreeStyle™ 293 medium (12338018, Gibco, Carlsbad, CA, USA) at 37 °C, 8% CO2, and 120 rpm. Human tumor cells A498, SKOV3, MDA-MB-468, A549, HepG2, BCPAP, and Raji were obtained from ATCC (Rockville, MD, USA) and stored in our lab. U-87 MG, PANC-1, MDA-MB-231, AsPC-1, and K562 were obtained from NCACC (Shanghai, China) and stored in our lab. Cells were maintained at 37 °C and 5% CO2. A498, U-87 MG, SKOV3, MDA-MB-468, PANC-1, A549, BCPAP, MDA-MB-231, HepG2, and AsPC-1 were cultured in DMEM (Hyclone, Waltham, MA, USA) supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin (Gibco, Carlsbad, CA, USA), and 10% fetal bovine serum (FBS) (Cegrogen, Stadtallendorf, Germany). K562, and Raji were maintained in RPMI 1640 (Hyclone, Waltham, MA, USA) with 100 U/mL penicillin, 100 µg/mL streptomycin, and 10% FBS.
3
Cultivation of Thyroid Cell Lines
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Normal thyroid cell line (Nthy-ori3-1) and thyroid carcinoma cell lines (TPC-1 and BC-PAP) were purchased from ATCC (Manassas, VA, USA). Cells were cultured in low-glucose Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (FBS) (Gibco, Rockville, MD, USA) and 1% penicillin-streptomycin.
4
Characterization of Thyroid Cell Lines
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The human thyroid cell line Nttry-ori-3-1 and PTC cell lines IHH4, BCPAP, K1 and TPC-1 were obtained from ATCC (Manassas, VA, USA). All cells were cultured in RPMI-1640 (Invitrogen, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/mL penicillin, and 0.1 mg/mL streptomycin in a humidified 5% CO2 incubator at 37°C and passaged at 80–90% confluence. The identity of the cells used in the experiment was confirmed using short tandem repeat analysis. All cell lines were tested for Mycoplasma every 3 months.
5
Culturing Human Thyroid Cell Lines
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Human thyroid cell line Nthy-ori 3–1 and THCA cell lines SW579, BCPAP, and TPC-1, purchased from ATCC (USA), were cultured in DMEM medium containing 10% FBS and incubated in an incubator containing 5% CO2 at 37 °C. Passage was performed when the confluence rate reached 90%.
6
Culturing Thyroid and HEK293 Cells
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The human thyroid normal cell line Nthy-ori 3-1; the PTC cell lines BCPAP, KTC-1, and K1; and HEK293 cells were purchased from ATCC (Manassas, VA, USA). Cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) and maintained in a humidified incubator with 5% CO2 at 37°C.
7
LPA Signaling Pathway Characterization
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CGTH‐W3 and TPC‐1 were obtained from Taizhou Central Hospital. B‐CPAP and BHT‐101 were purchased from ATCC. Cells were maintained in an appropriate medium as protocol and incubated in a humidified atmosphere of 95% air plus 5% CO2 at 37℃. TC LPA5 4 was purchased from Tocris Bioscience. Wortmannin was obtained from MCE. Rapamycin was purchased from LC Laboratories. LPA (18:1) was purchased from Avanti Polar‐Lipids. pEGFP‐C1‐Grp1‐PH was obtained from Addgene.
8
Culturing PTC and Normal Thyroid Cells
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Three PTC cell lines, TPC1, KTC1, and BCPAP, and normal thyroid epithelial cells, Nthy-ori3-1, were purchased from ATCC. TPC1 cells were cultured in a DMEM medium (Gibco, Carlsbad, CA, USA), while KTC1, BCPAP, and Nthy-ori3-1 were cultured in an RPMI1640 medium (Gibco, Carlsbad, CA, USA), both of which contained 10% fetal bovine serum (Gibco, Carlsbad, CA, USA). The cells were maintained in a humidified incubator at 37 °C and 5% CO2.
9
Culturing Human Thyroid Cancer Cell Lines
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Human thyroid cancer cell lines (TPC-1 and BCPAP) were obtained from ATCC (American Type Culture Collection) (Manassas, VA, USA), and cultured in DMEM (Dulbecco’s modified Eagle’s medium) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/mL penicillin and 100 μg/mL streptomycin (Hyclone, South Logan, UT, USA), and incubated at 37 °C in a humidified atmosphere containing 5% CO2.
10
Cell Culture of Thyroid Cell Lines
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Human thyroid normal cell line, Nthy-ori 3-1, PTC cell lines (BCPAP, KTC-1, and K1), and HKE 293 cells were purchased from ATCC company (ATCC, Manassas, USA). All the cells were cultured in the Dulbecco's modified Eagles' medium (DMEM, Invitrogen, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen), and maintained in a humidified incubator with 5% CO2 at 37°C.
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