Sip 22

Manufactured by Harvard Apparatus

Sourced in United States

The SIP 22 is a syringe infusion pump designed for precise fluid delivery in research and laboratory applications. It can accurately control the flow rate and volume of a wide range of syringe sizes. The SIP 22 features a high-resolution stepper motor and a user-friendly interface for easy programming and operation.

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Lab products found in correlation

Microfil mv 122, by Flow Tech (2 mentions) Sc 152, by Santa Cruz Biotechnology (1 mentions)

2 protocols using sip 22

Renal Microvascular Architectural Assessment

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Renal microvascular architecture was assessed using 3D micro CT, and images analyzed as previously described [26 (link)]. Kidneys were perfused with an intravascular radio-opaque silicone polymer (Microfil MV122, Flow Tech, Carver, MA, USA) under physiological pressure using a syringe infusion pump (SIP 22; Harvard Apparatus, Holliston, MA, USA) through a cannula (PE 190) ligated in a segmental renal artery. Perfused sections from the cortex and medulla were preserved in 10% buffered formalin, and subsequently prepared and scanned using a micro-CT scanner. Spatial density (number of vessels/tissue area) of cortical microvessels (diameters of 20–500 μm) and microvascular tortuosity (an index of vessel immaturity) were calculated using Analyze™. In addition, peritubular capillaries were counted in Hematoxylin and Eosin-stained slides at ×100, and expressed as number of capillaries per tubules [27 (link)], whereas renal expression of the pro-angiogenic factor vascular endothelial growth factor (VEGF) was measured by Western blotting (sc-152, Santa Cruz, 1:200) [28 (link)].

Ferguson C.M., Farahani R.A., Zhu X.Y., Tang H., Jordan K.L., Saadiq I.M., Lerman A., Lerman L.O, & Eirin A. (2021). Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles Elicit Better Preservation of the Intra-Renal Microvasculature Than Renal Revascularization in Pigs with Renovascular Disease. Cells, 10(4), 763.

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Quantifying Renal Microvascular Structure

Cited 1 time

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Under physiological pressure, one kidney from each pig was perfused with a radio-opaque silicone polymer (Microfil MV122, Flow Tech, Carver, MA), using a saline-filled cannula (PE 190) and a syringe infusion pump (SIP 22; Harvard Apparatus, Holliston, MA) until the polymer drained freely from a segmental vein. Perfused sections were preserved in 10% buffered formalin, and then scanned using a micro-CT scanner, which generates 3D images consisting of up to a billion cubic voxels, each 5μm to 25μm on a side, with isotropic spatial resolution. Images were reconstructed (20μm voxels), and spatial density of cortical and medullary microvessels (diameters 20–500μm) calculated using Analyze^™, as previously described (Zhu et al., 2004 (link); Favreau et al., 2010 (link)). Vessel tortuosity was assessed by the “connectivity index” (vessel length divided by the shortest distance between its ends) (Bentley et al., 2002 (link)). In addition, media-to-lumen ratio was calculated in randomly selected intra-renal vessels in α smooth muscle actin (SMA)-stained sections (DakoCytomation A/S, Glostrup, Denmark).

Eirin A., Hedayat A.F., Ferguson C.M., Textor S.C., Lerman A, & Lerman L.O. (2018). MITOPROTECTION PRESERVES THE RENAL VASCULATURE IN PORCINE METABOLIC SYNDROME. Experimental physiology, 103(7), 1020-1029.

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