For in utero electroporation44 (link),50 (link), pregnant ICR mice at embryonic day (E) 14–15 were deeply anesthetized with sodium pentobarbital (Somnopentil; Kyoritsu Seiyaku Co., Tokyo, Japan) injected intraperitoneally. Plasmids were dissolved in water. The pCAG plasmid vectors were used to knockdown the expression of CNTNAP2 or AHI1 in pyramidal neurons. The plasmids (0.5–2 µg/µl) were injected into either the left or the right lateral ventricle, or bilateral ventricles with a glass micropipette. Electric pulses (35 V for 50 ms, five to ten times at 950 ms intervals) were delivered through forceps-shaped electrodes (CUY650P5, Unique Medical Imada, Aichi, Japan) connected to an electroporator (CUY21, Nepa Gene, Chiba, Japan). Pups that expressed strong fluorescence in the prefrontal cortex of both hemispheres were used for behavior experiments.
Somnopentil
Manufactured by Kyoritsu Seiyaku
Sourced in Japan
Somnopentil is a laboratory instrument designed for sleep research. It measures and records various physiological parameters during sleep, including brain activity, eye movements, and muscle activity. The device provides objective data to support sleep studies and clinical assessments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using somnopentil
1
In utero electroporation for gene knockdown
2
Radiolabeled Acetic Acid and Anesthetic Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Both [1-13C]Acetic acid and glacial acetic acid, were purchased from Wako Pure
Chemical (Tokyo, Japan) and Racol from Otsuka Pharmaceutical Co., Ltd. (Tokyo, Japan).
Sodium pentobarbital (Somnopentil) was obtained from Kyoritsuseiyaku Corporation (Tokyo).
Distilled water for injection was purchased from Otsuka Pharmaceutical Factory, Inc.
(Tokushima, Japan).
Chemical (Tokyo, Japan) and Racol from Otsuka Pharmaceutical Co., Ltd. (Tokyo, Japan).
Sodium pentobarbital (Somnopentil) was obtained from Kyoritsuseiyaku Corporation (Tokyo).
Distilled water for injection was purchased from Otsuka Pharmaceutical Factory, Inc.
(Tokushima, Japan).
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