Pluronic f127

APM (460.5 Da, molecular weight, and 99.6% purity) was acquired from Wuhan Senwayer Century Chemical (Wuhan, China). Carbomer 940 (CAS 9003-01-4) and Pluronic F127 (CAS 9003-11-6) were obtained from Fagron (Waregem, Belgium). Hyaluronic acid (HA) with a molecular weight of 50 kDa was purchased from Cn Lab Canada, Asian Group (Xi’an, China). Sepigel 305^® (CAS 64742-47-8) (Polyacrylamide, C13-14 Isoparaffin Laureth-7) was obtained from SEPPIC. Diethylene glycol monoethyl ether (Transcutol-P) was provided by Gattefossé (Saint-Priest, France). Phosphate-buffered saline (PBS) tablets were purchased from Sigma (Darmstadt, Germany), processed according to the manufacturer’s instructions, and stored in the refrigerator for further use. The HaCaT cell line was acquired from Cell Line Services (Eppelheim, Germany), and reagents utilized for cell culture were purchased from Gibco (Carcavelos, Portugal). The reagents utilized for the MTT assay were purchased from Invitrogen Alfagene^® (Carcavelos, Portugal). Reagents used for the MTT assay were purchased from Invitrogen Alfagene^® (Carcavelos, Portugal). All the reagents for histological procedures were purchased from Sigma and Thermo Fisher Scientific (Barcelona, Spain). Milli-Q^® purified water was used in all experiments. To conclude, all reagents used in this study were of analytical grade.

Carbopol-940^® (Carbomer 940) and Pluronic F-127^® (Poloxamer 407) were obtained from Fagron Iberica (Barcelona, Spain). Stearic acid was provided by VWR Chemicals (Leuven, Belgium), and triethanolamine was purchased from Panreac AppliChem (Darmstadt, Germany). Glycerin was obtained from VWR Chemicals (Leuven, Belgium), and methylparaben was sourced from Merck (Burlington, MA, USA). Water was purified (Millipore^®) and filtered through a 0.22 mm nylon filter before use. All solvents used were HPLC grade, whereas all other materials were of analytical grade.