β actin antibody

Tissues or cells were lysed with RIPA buffer (Pierce, Rockford, IL) supplemented with protease inhibitors. Protein concentration was measured using a BCA protein assay (Thermo Scientific, Rockford, IL). Protein lysates, suspended in loading buffer, were separated on 10% SDS-polyacrylamide gels and transferred onto PVDF membranes (Millipore, Belford, MA). Then these membranes were blocked with 5% skim milk at room temperature for 1 h, and incubated with primary antibodies at 4 °C overnight. After washed, they were incubated with suitable HRP-conjugated secondary antibody at room temperature for 30 min and detected using an enhanced chemiluminescence (ECL) kit (Thermo Scientific). Antibodies for CASZ1, ERK and p-ERK were obtained from Abcam (Cambridge, MA), for RAF1, cyclinD1, E-cadherin, N-cadherin and Vimentin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), for MMP2 and MMP9 were purchased from Affinity Biosciences (Cincinnati, OH). β-actin antibody and corresponding secondary antibodies were purchased from Zhongshan Golden Bridge Biotechnology (ZSGB, Beijing, China).

High-salt (HS) feed containing 8% sodium chloride (NaCl) was purchased from Beijing Keao Xieli Feed Company (Beijing, China), taurine was purchased from Beijing Puboxin Biotechnology Company (Beijing, China), and horseradish peroxidase-labeled secondary antibodies, NaHS, and the CBS inhibitor hydroxylamine hydrochloride (HA) were purchased from Sigma-Aldrich (St. Louis, MO, USA) [30 (link)]. Antibodies against CBS , MPST, gp91phox, p47phox, p22phox and CD31 were purchased from Santa Cruz (Dallas, TX, USA); CSE antibody was purchased from Sigma-Aldrich; antibodies against CSAD, CDO1, CD31 and renin were purchased from Abcam (Waltham, MA, USA); β-actin antibody was purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China); GAPDH antibody was from Shanghai Kangcheng Biological Engineering Company (Shanghai, China), and antibodies against SOD1 and SOD2 were purchased from Enzo Life (Farmingdale, NY, USA).