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Fitc mouse anti human cd8

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The FITC Mouse Anti-Human CD8 is a flow cytometry reagent used for the identification and enumeration of human CD8-positive cells. It consists of a fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody that specifically binds to the CD8 surface antigen expressed on a subset of human T lymphocytes.

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Lab products found in correlation

Apc mouse anti human cd4, by BD (3 mentions) Percp cy5.5 mouse anti human cd19, by BD (2 mentions) Pe mouse anti human cd28, by BD (2 mentions) V450 mouse anti human cd194 ccr4, by BD (2 mentions) Alexa fluor 700 mouse anti human cd183 cxcr3, by BD (2 mentions) Fitc mouse anti human cd4, by BD (2 mentions) Mouse igg1 fitc, by BD (1 mentions) Pe mouse anti human cd14, by BD (1 mentions) Mouse igg2a pe, by BD (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Dspe peg mal, by Avanti Polar Lipids (1 mentions) Mouse anti human cd4 pe, by BD (1 mentions) Cy3 conjugated goat anti mouse igg, by Proteintech (1 mentions) Polyethylenimine (pei), by Merck Group (1 mentions) Hek293ft, by American Type Culture Collection (1 mentions) Facs lysing solution, by BD (1 mentions) Apc mouse anti human cd3, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Fitc mouse anti human cd45, by BD (1 mentions)

6 protocols using fitc mouse anti human cd8

1

Multicolor Flow Cytometry Immunophenotyping

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APC Mouse anti-Human CD4 (BD Biosciences), FITC Mouse Anti-Human CD8 (BD), PE Mouse Anti-Human CD28 (BD), V450 Mouse Anti-Human CD194 (CCR4) (BD), Alexa Fluor 700 Mouse anti-Human CD183 (CXCR3) (BD), and PerCP-Cy5.5 Mouse anti-Human CD19 (BD).
Moore B.B., Fry C., Zhou Y., Murray S., Han M.K., Martinez F.J, & Flaherty K.R. (2014). Inflammatory Leukocyte Phenotypes Correlate with Disease Progression in Idiopathic Pulmonary Fibrosis. Frontiers in Medicine, 1, 56.
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2

Nanoparticle Synthesis and Characterization

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PLGA (75:25 L:G; ester-terminated, inherent viscosity range: 0.55–0.75 dL/g in CHCl3) was purchased from Lactel. All lipids for the nanoparticle synthesize were purchased from Avanti Polar Lipids, including 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG-MAL). Cholesteryl butyrate was purchased from Santa Cruz Biotechnology. Rhesus recombinant anti-CD4 antibody and rhesus recombinant IgG1 isotype control antibody were purchased from NIH Nonhuman Primate Reagent Resource. FITC mouse anti-human CD8, PE mouse anti-human CD14, PerCP mouse anti-human CD3 antibodies, FITC mouse anti-human CD4, and FITC mouse IgG1, PE mouse IgG2a, PerCP mouse IgG1 κ control antibodies were purchased from BD Biosciences. RPMI 1640 containing 2 mMl-glutamine and 25 mM HEPES, DPBS, heat-inactivated fetal bovine serum (FBS), Penicillin–Streptomycin (10,000 U/mL), 1,1′ -Dioctadecyl-3,3,3′, 3′ -Tetramethylin-dodicarbocyanine, 4-Chlorobenzenesulfonate Salt (DiD), LIVE/DEAD® Fixable Violet Dead Cell Stain Kit, Dylight 633 NHS Ester were purchased from ThermoFisher. All other chemicals were purchased from Sigma-Aldrich and Fisher Scientific unless otherwise specified.
Cao S., Jiang Y., Levy C.N., Hughes S.M., Zhang H., Hladik F, & Woodrow K.A. (2018). Optimization and comparison of CD4-targeting lipid–polymer hybrid nanoparticles using different binding ligands. Journal of biomedical materials research. Part A, 106(5), 1177-1188.
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3

Multicolor Flow Cytometry Immunophenotyping

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APC Mouse anti-Human CD4 (BD Biosciences), FITC Mouse Anti-Human CD8 (BD), PE Mouse Anti-Human CD28 (BD), V450 Mouse Anti-Human CD194 (CCR4) (BD), Alexa Fluor 700 Mouse anti-Human CD183 (CXCR3) (BD), and PerCP-Cy5.5 Mouse anti-Human CD19 (BD).
Moore B.B., Fry C., Zhou Y., Murray S., Han M.K., Martinez F.J, & Flaherty K.R. (2014). Inflammatory Leukocyte Phenotypes Correlate with Disease Progression in Idiopathic Pulmonary Fibrosis. Frontiers in Medicine, 1, 56.
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4

Cell Line Culture and Transfection

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HEK293FT, FaDu, and Hela cell lines were purchased from the American Type Culture Collection. The cells were cultured with Dulbecco’s modified Eagle medium with 10% fetal bovine serum and 100 μg/mL penicillin–streptomycin. Polyethylenimine (PEI) used as a cell transfection reagent was purchased from Sigma (St. Louis, MO, USA). Antibodies used in this study included Cy3-conjugated goat anti-mouse IgG (ProteinTech, SA0009-1), PE mouse anti-human CD4 (BD Pharmingen, 55347), and FITC mouse anti-human CD8 (BD Pharmingen, 555366).
Zhong K., Liu Z., Li H., Zhao S., Wang Y., Guo W., Zheng X., Yang H., Guo G., Zhou L., Xu J, & Tong A. (2020). T cell stimulation and expansion by SunTag-based clustering of anti-CD3/CD28 scFv. Aging (Albany NY), 12(11), 11061-11070.
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5

Multicolor Flow Cytometry Analysis of T-Cell Subsets

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100 μl of EDTA-anticoagulated peripheral blood was stained with 5 μl of the following fluorochrome-conjugated antibodies: anti-human CD3-APC (BD Pharmingen™ APC Mouse Anti-Human CD3), anti-human CD279-PE (BD Pharmingen™ PE Mouse Anti-Human CD279) and anti-human CD4-FITC (BD Pharmingen™ FITC Mouse Anti-Human CD4) or anti-human CD8-FITC (BD Pharmingen™ FITC Mouse Anti-Human CD8) and incubated for 30 minutes at 4 °C in dark. Next, the cells were lysed by the BD FACS Lysing Solution (Becton Dickinson) for 15 minutes at 4 °C, and washed twice with cold PBS. Isotype control antibodies: anti-human CD3, anti-human CD279 and anti-human CD4 or anti-human CD8 were used for setting compensation and to set gates. Specimen assays were performed using the BD LSRFortessa (BD Biosciences) and the data were analyzed by FlowJo 10.0.7 software.
Shen T., Zhou L., Shen H., Shi C., Jia S., Ding G.P, & Cao L. (2017). Prognostic value of programmed cell death protein 1 expression on CD8+ T lymphocytes in pancreatic cancer. Scientific Reports, 7, 7848.
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6

Multiparametric T-cell Phenotyping

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We used fluorochrome-conjugated antibodies to MSLN (anti-MSLN PE-conjugated Rat IgG2A FAB32652P; R&D Systems), CD3 (PE/Cy7 anti-human CD3 Clone HI3A, 300316; Biolegend), CD45 (FITC Mouse antihuman CD45, 555482; BD Pharmingen), CD4 (APC Mouse Anti-Human CD4, 55349; BD Pharmingen), CD8 (FITC Mouse Anti-Human CD8, 561948; BD Pharmingen), CD62L, CD45RA, and CXCR3 (AF700 mouse antihuman CXCR3, 353742; Biolegend) (Supplementary Table S1). In assessing in vitro and in vivo T-cell proliferation and accumulation, cells were labeled with either CFSE or CellTrace Violet cell proliferation stain, in accordance with the manufacturers' instructions. Cells were quantified using CountBright Absolute Counting Beads (Life Technologies). All flow cytometric analyses were performed on either a BD FACSCalibur or LSR II (BD Biosciences, San Jose, CA); data were analyzed using FlowJo software (version 6.0, TreeStar).
Quach H.T., Skovgard M.S., Villena-Vargas J., Bellis R.Y., Chintala N.K., Amador-Molina A., Bai Y., Banerjee S., Saini J., Xiong Y., Vista W.R., Byun A.J., De Biasi A., Zeltsman M., Mayor M., Morello A., Mittal V., Gomez D.R., Rimner A., Jones D.R, & Adusumilli P.S. (2023). Tumor-Targeted Nonablative Radiation Promotes Solid Tumor CAR T-cell Therapy Efficacy. Cancer immunology research, 11(10).
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