Fitc labeled anti guinea pig igg for mnv 1

GCDCA and chloroquine were purchased from Sigma-Aldrich (St Louis, MO). Cathepsin L inhibitors, Z-FY-CHO and MDL28170, were purchased from Santa Cruz Biotech (Santa Cruz, CA), and a cathepsin B inhibitor CA074-Me was purchased from Sigma-Aldrich. Recombinant human cathepsin L and cathepsin B were purchased from R & D systems (Minneapolis, MN). The primary antibodies used in this study were anti-PEC/Cowden antibodies raised in swine (Chang et al., 2005 (link)), anti-PEC VP2 antibodies raised in guinea pig (Chang et al., 2005 (link)), anti-FCV antibodies raised in guinea pig (Sosnovtsev and Green, 1995 (link)), anti-MNV-1 antibodies raised in guinea pig (Wobus et al., 2004 (link)) and anti-Rab7 antibodies (Santa Cruz Biotech, CA). The secondary antibodies for Western blot analysis included horseradish peroxidase-conjugates of anti-swine Ig and anti-guinea pig Ig antibody (Thermo Scientific, Pittsburgh, PA). The secondary antibodies for confocal microscopy were FITC-labeled anti-swine IgG for PEC (Kirkegaard & Perry Lab Inc, MD), FITC-labeled anti-guinea pig IgG for MNV-1 (Sigma-Aldrich, MO) and anti-rabbit PerCP-Cy 5.5 (Santa Cruz Biotech, CA). Other basic chemicals for confocal microscopy and other studies were purchased from various sources including Sigma-Aldrich.