Alkaline phosphatase conjugated antibodies

Manufactured by Promega

Sourced in Canada

Alkaline phosphatase‐conjugated antibodies are a type of lab equipment that can be used to detect and quantify target molecules in various assays. The alkaline phosphatase enzyme is covalently attached to the antibody, allowing for the visualization and amplification of the target signal.

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Lab products found in correlation

3 protocols using alkaline phosphatase conjugated antibodies

Antibody Sources for Western Blot

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Primary antibodies were obtained from the following sources: Na⁺/K⁺ ATPase‐α, calnexin, calpain 2, CDK2, cyclin B1, cyclin E, ERK2, lamin B, HA tag, p21, p27^Kip1, p57^Kip2, β‐tubulin from Santa Cruz Biotechnology (Santa Cruz, CA), β‐actin from Chemicon International (Billerica, MA), phospho‐AKT (Ser473), AKT, phosphorylated ERK1/2 (T202/Y204), and Lamp1 from Cell Signaling Technology (Danvers, MA) and Cathepsin B from R&D Systems (Mississauga, ON, Canada). Mouse and rabbit horseradish peroxidase antibodies were purchased from Amersham Biosciences (Pittsburg, PA), goat horseradish peroxidase antibody from Santa Cruz, and alkaline phosphatase‐conjugated antibodies were purchased from Promega (Madison, WI).

Bian B., Mongrain S., Cagnol S., Langlois M., Boulanger J., Bernatchez G., Carrier J.C., Boudreau F, & Rivard N. (2015). Cathepsin B promotes colorectal tumorigenesis, cell invasion, and metastasis. Molecular Carcinogenesis, 55(5), 671-687.

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Antibody Validation and Wnt Pathway Analysis

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Primary antibodies were obtained from the following sources: β-actin (MAB1501R) and phosphorylated ERK1/2 (M8159) from Millipore Sigma (Oakville, ON, Canada), ERK2 (sc-154) from Santa Cruz Biotechnology (Santa Cruz, CA, USA), non-phospho (active) β-catenin (D13A1), LRP5 (D80F2), LRP6 (C5C7), LRP6 (C47E12) and phospho-LRP6 (Ser1490) from Cell Signaling Technology (Danvers, MA, USA) and β-catenin (C14) and BrdU (B44) from BD Biosciences (San Jose, CA, USA). Horseradish peroxidase antibodies were obtained from GE Healthcare Life Sciences (Mississauga, ON, Canada) and alkaline phosphatase-conjugated antibodies from Promega Corporation (Madison, WI, USA). The specific ERK inhibitor SCH772984 was obtained from Cayman Chemical Company (Ann Arbor, MI, USA). Recombinant mouse Wnt3a was obtained from Abcam (Cambridge, MA, USA). Other materials were purchased from Millipore Sigma unless stated otherwise.

Raisch J., Côté-Biron A., Langlois M.J., Leblanc C, & Rivard N. (2021). Unveiling the Roles of Low-Density Lipoprotein Receptor-Related Protein 6 in Intestinal Homeostasis, Regeneration and Oncogenesis. Cells, 10(7), 1792.

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Immunoblotting and Immunofluorescence Protocols

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Primary antibodies were obtained from the following sources: DUSP6 (ab76310) from Abcam (Toronto, Canada), β‐actin (MAB1501R) from Millipore (Oakville, Canada), ERK2 (sc‐154) and MEK2 (sc‐524) from Santa Cruz Biotechnology (Santa Cruz, CA), phosphorylated ERK1/2 (M8159) from Sigma‐Aldrich (Oakville, Canada), phosphorylated MEK1/2 (9121) from Cell Signaling Technology (Danvers, MA, USA), chromogranin A (20085) from ImmunoStar (Hudson, WI), proliferating cell nuclear antigen (PCNA; 18197) from Abcam and Ki67 (GTX16667) from Genetex (Irvine, CA). Horseradish peroxidase antibodies were obtained from GE Healthcare Life Sciences (Mississauga, Canada) and alkaline phosphatase‐conjugated antibodies from Promega (Madison, WI). For immunofluorescence, Alexa Fluor 488 conjugated antibodies were obtained from Molecular Probes (Waltham, MA). Other materials were purchased from Sigma‐Aldrich unless stated otherwise.

Beaudry K., Langlois M., Montagne A., Cagnol S., Carrier J.C, & Rivard N. (2018). Dual‐specificity phosphatase 6 deletion protects the colonic epithelium against inflammation and promotes both proliferation and tumorigenesis. Journal of Cellular Physiology, 234(5), 6731-6745.

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