Ire1α 3294

Manufactured by Cell Signaling Technology

Sourced in United States

IRE1α (3294) is a recombinant protein that corresponds to the cytoplasmic domain of human inositol-requiring enzyme 1 alpha (IRE1α). IRE1α is a serine/threonine protein kinase and endoribonuclease that plays a central role in the unfolded protein response (UPR) pathway.

Automatically generated - may contain errors

Lab products found in correlation

Chop 2895, by Cell Signaling Technology (1 mentions) Flagellin, by InvivoGen (1 mentions) Tet plko puro, by Addgene (1 mentions) Uric acid, by Merck Group (1 mentions) Poly 1 c, by InvivoGen (1 mentions) Dmso 472301, by Merck Group (1 mentions) Perk 3192, by Cell Signaling Technology (1 mentions) Forskolin f6886, by Merck Group (1 mentions) Plko 1, by Addgene (1 mentions) Hcv core, by Thermo Fisher Scientific (1 mentions) P38 mapk 9212, by Cell Signaling Technology (1 mentions) Perk 5683, by Cell Signaling Technology (1 mentions) Jnk 9252, by Cell Signaling Technology (1 mentions) Phospho ire1α pa1 16927, by Thermo Fisher Scientific (1 mentions) Phospho perk ma5 15033, by Thermo Fisher Scientific (1 mentions)

3 protocols using ire1α 3294

Evaluating Endoplasmic Reticulum Stress

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The antibodies used for Western blot are, GRP78/HSPA5 (11587-1-AP), PERK/EIF2AK3 (24390-1-AP), and ATF6 (24169-1-AP) from Proteintech, Rosemont, IL, USA. IRE1α (3294) and CHOP (2895) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Samanta S., Tamura S., Dubeau L., Mhawech-Fauceglia P., Miyagi Y., Kato H., Lieberman R., Buckanovich R.J., Lin Y.G, & Neamati N. (2020). Clinicopathological significance of endoplasmic reticulum stress proteins in ovarian carcinoma. Scientific Reports, 10, 2160.

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Inhibitor-based Stress Response Assay

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DMSO (472301), tunicamycin (T7765), thapsigargin (T9033), brefeldin A (B7651), actinomycin D (A1410), DRB (D1916) and Forskolin (F6886) were purchased from Sigma. Monosodium urate crystals were prepared from uric acid (U0881, Sigma) as previously described[30 (link)]. PolyI:C, flagellin, MDP and R837 were from Invivogen. All siRNA were purchased from Ambion. Different shRNA were cloned in pLKO.1 (Addgene #10878) and/or in Tet-pLKO-puro (Addgene #21915) vectors as previously described[31 (link)]. A complete list of siRNA and shRNA used is presented in S1 Table. IRE1α (#3294) and PERK (#3192) antibodies were from Cell Signaling Technology, ATF6 antibody (ab122897) was from Abcam, and XBP-1s antibody (clone 143F) was from Biolegend. ATF4 antibody for ChIP (sc-22800) was from Santa Cruz. NLRP1 antibody (AF6788) was from R&D Systems.

D’Osualdo A., Anania V.G., Yu K., Lill J.R., Kaufman R.J., Matsuzawa S.I, & Reed J.C. (2015). Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress. PLoS ONE, 10(6), e0130635.

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Antibody Optimization for Cell Stress Markers

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Each of the primary antibodies were used according to the manufacturer’s protocol: Phospho-JNK #4671, JNK #9252, IRE1α #3294, PERK #5683, phospho-p38 MAPK #9215, p38 MAPK #9212, phospho-p44/42 MAPK #9106, p44/42 MAPK #4695, (Cell Signaling, Danvers, MA). phospho- IRE1α #PA1-16927, phospho-PERK #MA5-15033, and HCV core (Thermo Scientific, Rockford, IL). ATF6 #ab11909 (Abcam, Cambridge, MA).

Chusri P., Kumthip K., Hong J., Zhu C., Duan X., Jilg N., Fusco D.N., Brisac C., Schaefer E.A., Cai D., Peng L.F., Maneekarn N., Lin W, & Chung R.T. (2016). HCV induces transforming growth factor β1 through activation of endoplasmic reticulum stress and the unfolded protein response. Scientific Reports, 6, 22487.

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