5–10 head samples were treated with lysis buffer (TBS1x, 150mMNaCl, IP 50x) and then homogenized and centrifuged 13500 rpm for 5 minutes. After selecting the supernatant we added NuPage 4x (Invitrogen by Thermo Fisher Scientific) and ß-mercaptoethanol 5%. Western blot analysis samples were run in a 4%–12% gradient SDS-PAGE for the detection of sHSP23 and sHSP26. After electro-blotted onto nitrocellulose 0.45 μM (GE Healthcare) 100V for 1 hour, we blocked the membranes in TBS-Tween-20 buffer with 5% BSA. We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000) (Abmart) We visualized the antibody-protein interaction by chemo luminescence using IRDye Secondary Antibodies anti-mouse (IRDye 800CW, LI-COR), anti-rabbit (IRDye 680 RD, LI-COR) and developed with Odyssey equipment. Tubulin were used as a control.
Anti hsp23 antibody
Manufactured by Merck Group
The Anti-Hsp23 antibody is a laboratory reagent used to detect and quantify the presence of the Hsp23 protein in biological samples. Hsp23 is a heat shock protein that plays a role in cellular stress response. The antibody specifically binds to Hsp23, allowing researchers to measure its expression levels or localize the protein within cells or tissues.
Automatically generated - may contain errors
2 protocols using anti hsp23 antibody
1
Quantification of sHSP23 and sHSP26 Proteins
2
Protein Expression Analysis via Western Blot
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5-10 head samples were treated with lysis buffer (TBS1x, 150mMNaCl, IP 50x) and then homogenized and centrifuged 13500 rpm for 5 minutes. After selecting the supernantant we added NuPage 4x (Invitrogen by Thermo Fisher Scientific) and ßmercaptoethanol 5%.Western blot analysis samples were run in a 4%-12% gradient SDS-PAGE for the detection of sHSP23 and sHSP26. After electro-blotted onto nitrocellulose 0.45 μM (GE Healthcare) 100V for 1 hour, we blocked the membranes in TBS-Tween-20 buffer with 5% BSA. We incubated the membranes overnight at 4ºC in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000) (Abmart) We visualized the antibody-protein interaction by chemoluminescence using IRDye Secondary Antibodies anti-mouse (IRDye 800CW, LI-COR), anti-rabbit (IRDye 680 RD, LI-COR) and developed with Odyssey equipment.
Tubuline were used as a control.
Tubuline were used as a control.
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