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Ti2 e microscope

Manufactured by Hamamatsu Photonics
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The Ti2-E microscope is a high-performance inverted fluorescence microscope designed for advanced cellular and tissue imaging. It features a stable and vibration-free optical system, allowing for high-resolution imaging of a wide range of samples. The microscope is equipped with a high-sensitivity camera and a comprehensive range of objectives to accommodate various experimental requirements.

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Lab products found in correlation

Perfect focus system, by Nikon (2 mentions) Orca flash 4.0 camera, by Hamamatsu Photonics (2 mentions) Laser module, by Oxford Instruments (1 mentions) Eclipse ti microscope, by Yokogawa (1 mentions) Du897 emccd camera, by Oxford Instruments (1 mentions) Csu x1 spinning disk, by Agilent Technologies (1 mentions) Fusionbt scmos camera, by Hamamatsu Photonics (1 mentions) Csu w1 spinning disk, by Hamamatsu Photonics (1 mentions) Orca flash 4.0 scmos camera, by Hamamatsu Photonics (1 mentions) Thunder imaging system, by Leica camera (1 mentions) 700 confocal microscope, by Zeiss (1 mentions) Csu w1 sora, by Nikon (1 mentions) Nis elements ar 5, by Nikon (1 mentions)

5 protocols using ti2 e microscope

1

Timelapse Imaging of Cell Lines

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Timelapse imaging of NIH3T3 cells, SYF cells, and HEK293T cells was performed on a Nikon Eclipse Ti microscope with a Yokogawa CSU-X1 spinning disk, an Agilent laser module containing 405, 488, 561, and 650 nm lasers, and an iXon DU897 EMCCD camera, using ×40 or ×60 oil objectives. Timelapse imaging of MCF10A cells on polyacrylamide substrata was performed on a Nikon Ti2-E microscope with a CSU-W1 SoRa spinning disk, a Hamamatsu FusionBT sCMOS camera, using a ×20 air objective with ×2.8 magnification optics.
Farahani P.E., Yang X., Mesev E.V., Fomby K.A., Brumbaugh-Reed E.H., Bashor C.J., Nelson C.M, & Toettcher J.E. (2023). pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cells. eLife, 12, e82863.
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2

Live Cell Imaging of 96-well Plates

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Cells seeded into a 96 well plate (MGB096–1-2-LG-L) were imaged in DMEM on a Nikon Ti2-E microscope with a CSU-W1 spinning disk, Hamamatsu ORCA-FLASH 4.0 sCMOS camera, and 20x objective (NA 0.75) at 37°C, 5% CO2, and humidity. Twelve sites were imaged per well every hour for 24 hours in triplicate wells.
Le Guerroué F., Bunker E.N., Rosencrans W.M., Nguyen J.T., Basar M.A., Werner A., Chou T.F., Wang C, & Youle R.J. (2023). TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1. Molecular cell, 83(6), 927-941.e8.
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3

Confocal and Super-Resolution Microscopy

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Images were acquired on an inverted Zeiss 700 confocal microscope equipped with a motorized stage, 405/488/561 laser lines, and a 40X/1.3NA lens; or on an inverted Leica Thunder imaging system with individual LED lines, and 20X/0.8NA or 63 X/1.4NA lenses. Confocal images were acquired at 8-bit. All thunder images were acquired at 16-bit, subjected to computational clearing, and then set to the same fluorescence range (eg. 0–1000) within analysis cohorts before converting to 8-bit in Fiji. Representative super-resolution images in Figure 2G,2H, and 3A were acquired with 4X Super Resolution by Optical Pixel Reassignment (SoRa) on an inverted spinning disk confocal microscope (Yokogawa CSU-W1 SoRa/Nikon Ti2-E Microscope) equipped with Hamamatsu Fusion BT cameras, and 20X water (0.95 NA. WD 1.0 mm) or 60X oil (1.49 NA. WD 0.14 mm) immersion lenses. All images within analysis cohorts were acquired using identical acquisition parameters or processed identically to maintain relative differences in fluorescence, and underwent native deconvolution and denoising using NIS-Elements Software.
Pannoni K., Gil D., Cawley M., Alsalman M., Campbell L, & Farris S. (2023). Layer-specific mitochondrial diversity across hippocampal CA2 dendrites. Hippocampus, 33(3), 182-196.
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4

Time-lapse Fluorescence Microscopy

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Images were recorded using a Nikon Ti2-E microscope equipped with a ×60 objective and a Hamamatsu Orca-Flash 4.0 camera. The microscope was operated using Nikon NIS Elements AR 5.21.03 64-bit software and the objective’s axial position was controlled by the Nikon Perfect Focus System. To reduce photobleaching of the fluorescent protein, images were taken every 10 min with 100-ms exposure time.
Gligorovski V., Sadeghi A, & Rahi S.J. (2023). Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor. Nature Communications, 14, 3810.
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5

Yeast Cell Fluorescence Quantification

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Images were recorded using a Nikon Ti2-E microscope equipped with a 60x objective and a Hamamatsu Orca-Flash 4.0 camera. The microscope was operated using NIS-Elements software and the objective's axial position was controlled by Nikon Perfect Focus System. To prevent photobleaching, images were taken every 10 min with 100 ms exposure time.
Image analysis was performed using YeaZ, a Python-based tool for yeast cell segmentation 19 (link) . First, the boundaries of cells were determined in phase-contrast images. The levels of fluorescence for each cell were calculated as an average of the pixel intensities in the yellow fluorescence channel for pixels that were within the cell boundaries. For further analyses, we subtracted the autofluorescence of unlabeled wild-type cells from the fluorescence values.
Gligorovski V., Sadeghi A., & Rahi S.J. (2020). Multidimensional single-cell benchmarking of inducible promoters for precise dynamic control in budding yeast.
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