Species specific hrp conjugated secondary antibodies

Manufactured by Merck Group

Species-specific HRP conjugated secondary antibodies are a type of laboratory reagent used in various immunoassay techniques. They are designed to detect and bind to primary antibodies raised against specific target species, with horseradish peroxidase (HRP) enzyme conjugated to facilitate detection.

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Lab products found in correlation

Hybond nitrocellulose membrane, by Cytiva (2 mentions) Hpv18 e6 g 7, by Santa Cruz Biotechnology (2 mentions) Gapdh g 9, by Santa Cruz Biotechnology (2 mentions) Phospho erk1 2, by Cell Signaling Technology (2 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (2 mentions) Phospho gsk3α β, by Cell Signaling Technology (1 mentions) Ha ha 7, by Merck Group (1 mentions) Frs2α, by Cell Signaling Technology (1 mentions) Phospho akt ser473 d9e, by Cell Signaling Technology (1 mentions) Cyclin b1 h 433, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) α sma, by Abcam (1 mentions) Clic4, by Santa Cruz Biotechnology (1 mentions) C jun, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Hpv18 e7, by Abcam (1 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Specific HRP-conjugated secondary antibodies HRP-conjugated antibodies Specific antibodies

3 protocols using species specific hrp conjugated secondary antibodies

Protein Expression Analysis in Keratinocytes

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Total protein was extracted from keratinocytes in Leeds lysis buffer [56 (link)] and resolved by SDS-PAGE (10-15% Tris-Glycine), transferred onto Hybond nitrocellulose membrane (Amersham biosciences) and probed with antibodies specific for phosphorylated FRS2α (3861, Cell Signaling Technology), cyclin B1 (H-433, Santa Cruz Biotechnology), involucrin (SY5, Santa Cruz Biotechnology), HPV18 E6 (G-7, Santa Cruz Biotechnology), HPV18 E7 (8E2, Abcam (ab100953), AKT (9272, Cell Signaling Technology), phospho-AKT Ser473 (D9E, Cell Signaling Technology), HA (HA-7, Sigma H9658), cytokeratin 1 (Poly19056, Covance), phospho-ERK1/2 (43705, Cell Signalling Technology), GAPDH (G-9, Santa Cruz Biotechnology), phospho-GSK3α/β (9336, Cell Signalling Technology) and EGFR (R-1, Santa Cruz Biotechnology). Immunoblots were visualized with species-specific HRP conjugated secondary antibodies (Sigma) and ECL (Thermo/Pierce).

Wasson C.W., Morgan E.L., Müller M., Ross R.L., Hartley M., Roberts S, & Macdonald A. (2017). Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget, 8(61), 103581-103600.

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Profiling Fibroblast Protein Expression

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Total proteins were extracted from the fibroblasts in RIPA buffer and resolved by SDS-PAGE (10–15% Tris-Glycine). The proteins were transferred onto nitrocellulose membranes (Amersham biosciences) and probed with antibodies that were specific for α-SMA (Abcam), CLIC4 (Santa Cruz), GLI1 (Santa Cruz), GLI2 (R&D systems), SMAD3, β-catenin, c-Jun (Cell signalling), and β-Actin (Sigma). The immunoblots were visualized with species-specific HRP conjugated secondary antibodies (Sigma) and ECL (Thermo/Pierce) on a Biorad ChemiDoc imaging system. Densitometry analysis of the blots was performed using the ImageJ software.

Wasson C.W., Caballero-Ruiz B., Gillespie J., Derrett-Smith E., Mankouri J., Denton C.P., Canettieri G., Riobo-Del Galdo N.A, & Del Galdo F. (2022). Induction of Pro-Fibrotic CLIC4 in Dermal Fibroblasts by TGF-β/Wnt3a Is Mediated by GLI2 Upregulation. Cells, 11(3), 530.

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Protein Extraction and Western Blot Analysis

Cited 1 time

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Total protein was extracted from keratinocytes in lysis buffer (Mankouri et al., 2010 (link)) and resolved by SDS-PAGE (10–15% Tris-Glycine), transferred onto Hybond nitrocellulose membrane (Amersham biosciences) and probed with antibodies specific for cyclin B1 (H-433, Santa Cruz Biotechnology), HPV18 E6 (G-7, Santa Cruz Biotechnology), HPV18 E7 (8E2, Abcam (ab100953), phospho-ERK1/2 (43705, Cell Signalling Technology), GFP (sc-9996, Santa Cruz Biotechnology) and GAPDH (G-9, Santa Cruz Biotechnology). Immunoblots were visualized with species-specific HRP conjugated secondary antibodies (Sigma) and ECL reagent (Thermo/Pierce).

Wetherill L.F., Wasson C.W., Swinscoe G., Kealy D., Foster R., Griffin S, & Macdonald A. (2018). Alkyl-imino sugars inhibit the pro-oncogenic ion channel function of human papillomavirus (HPV) E5. Antiviral Research, 158, 113-121.

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