Liquid chromatography mass spectrometry lc ms grade acetonitrile

Sinigrin potassium salt (≥95.0%), imidazole formate (≥94%), DEAE Sepharose CL-6B suspension and sulfatase (from Helix pomatia, Type H-2, aqueous solution, ≥2000 units/mL), and certified rapeseeds (ERM-BC367) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The sulfatase was diluted 100-fold with ultrapure water to a working solution. Formic acid and liquid chromatography-mass spectrometry (LC-MS) grade acetonitrile were purchased from Fisher Scientific (Waltham, MA, USA). Ultrapure water was prepared from distilled water using a Milli-Q system (Millipore Lab., Bedford, MA, USA). All other chemicals and reagents were of analytical grade.

Amitriptyline hydrochloride (AMI-HCl), Brij™ O20, dichloromethane (DCM), anhydrous acetone, and sodium carbonate were obtained from Sigma-Aldrich (UK). Sodium dihydrogen phosphate, sodium hydrogen phosphate, magnesium sulphate, and dithiothreitol (DTT) were supplied by Fisher Scientific (UK). Phosphate-buffered saline (pH 7.3 ± 0.2 at 25 °C) was prepared using Dulbecco A tablets supplied by Oxoid (UK). Octyl salicylate (OSAL), PG, IPM, IPA, and 1-octanol were obtained from Sigma-Aldrich (UK). High-performance liquid chromatography (HPLC) grade water, HPLC grade acetonitrile, HPLC grade methanol, liquid chromatography/mass spectrometry (LC-MS) grade acetonitrile, LC-MS grade 0.1% trifluoroacetic acid (TFA) solution, limonene (LIM), ethyl acetate (EtAc), and TFA were supplied by Fisher Scientific (UK). Chloroform-d (CDCl₃) were obtained from Cambridge Isotope Laboratories Inc. (UK). Propylene glycol monolaurate (PGML) Type II and TC were gifts from Gattefossé (St. Priest, France). All chemicals, unless otherwise stated, were ≥ 99% purity.