Dulbecco’s Modified Eagle Medium (DMEM), Fetal Bovine Serum (FBS), and penicillin-streptomycin were purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Potassium persulfate, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and Folin & Ciocalteu’s phenol reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carboxy-H2DCFDA was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA), while superoxide dismutase (SOD) and catalase assay kits were obtained from Cayman (Cayman Chemical Company, Ann Arbor, MI, USA).
Catalase assay kit
Manufactured by Cayman Chemical
Sourced in United States, United Kingdom
The Catalase Assay Kit is a laboratory tool used to measure the activity of the enzyme catalase. Catalase is an important antioxidant enzyme found in living organisms that helps break down hydrogen peroxide into water and oxygen. The kit provides the necessary reagents and protocols to quantify catalase levels in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Superoxide dismutase assay kit, by Cayman Chemical (32 mentions)
Glutathione peroxidase assay kit, by Cayman Chemical (13 mentions)
Sod assay kit, by Cayman Chemical (8 mentions)
Glutathione assay kit, by Cayman Chemical (5 mentions)
Glutathione reductase assay kit, by Cayman Chemical (4 mentions)
Gpx assay kit, by Cayman Chemical (4 mentions)
Glutathione peroxidase kit, by Cayman Chemical (3 mentions)
Antioxidant assay kit, by Cayman Chemical (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Potassium persulfate, by Merck Group (2 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (2 mentions)
Thiobarbituric acid reactive substances assay kit, by Cayman Chemical (2 mentions)
Imark microplate reader, by Bio-Rad (2 mentions)
Glutathione peroxidase activity colorimetric assay kit, by Abcam (2 mentions)
Mda assay kit, by Cayman Chemical (2 mentions)
Gsh assay kit, by Cayman Chemical (2 mentions)
Superoxide dismutase activity assay kit, by Abcam (2 mentions)
Tbars assay kit, by Cayman Chemical (2 mentions)
Gr assay kit, by Cayman Chemical (2 mentions)
Protein assay kit, by Bio-Rad (2 mentions)
126 protocols using catalase assay kit
1
Antioxidant Assays in Cell Culture
2
Antioxidant Response Evaluation in Cell Cultures
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Bovine serum albumin (BSA) was obtained from BBI Solutions (Cardiff, UK). DHA, EPA and catalase assay kits were purchased from Cayman (Ann Arbor, MI, USA). Low‐glucose Dulbecco's modified Eagle's medium, tert‐butyl hydroperoxide (tBHP) and MTT were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Fetal bovine serum was purchased from Gibco (Poisley, UK). Anti‐Ho‐1 antibody was purchased from Assay Design (Ann Arbor, MI, USA). Anti‐Nqo1 antibody was obtained from Abcam (Cambridge, UK). Anti‐Nrf2 antibody, anti‐β‐actin and anti‐lamin A/C antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The glutathione assay kit was purchased from OxisResearch (Foster City, CA, USA). CellROX Deep Red reagent was purchased from Invitrogen (Carlsbad, CA). Other reagents and chemicals were obtained from standard suppliers.
3
Catalase Activity Quantification
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Catalase activity levels were determined with commercial Catalase Assay Kits (Catalogue no. 707002-Cayman Chemical Company, USA) according to the manufacturer’s instructions. This assay is based on the reaction of catalase with methanol in the presence of hydrogen peroxide. The formaldehyde produced is measured upon its reaction with Purpald chromogen which specifically forms a bicyclic compound that changes to colorless to purple color upon oxidation. Catalase activity was measured using total proteins extract as described above and was calculated in nmol.min/μg of protein. To normalize the enzyme activity, the concentration of total protein extract was used. The experiment was performed in three biological replicates.
4
Antioxidant Enzyme Activities in Testis
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Testicular tissues (100 mg) were washed with 1X PBS (pH 7.4) to remove excess blood thoroughly. The activity of SOD, GPx, and catalase in whole tissue supernatant was evaluated using commercially available kits (Cayman Chemical, Ann Arbor, MI, USA; item no.706002, superoxide dismutase kit; item no. 703102, glutathione peroxidase kit; item no. 707002, catalase assay kit) as per manufacturer’s instructions. Values were expressed as milligrams of protein.
5
Catalase Activity Assay Protocol
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Homogenates suspended in 50 mM potassium orthophosphate buffer (pH 7.0) containing 1 mM EDTA were centrifuged at 10,000× g for 15 min at 4 °C. The supernatants were collected for enzyme activity determination using the Catalase Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) according to the instructions provided by the manufacturer.
6
Antioxidant Enzyme Measurement in Tissues
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Colon and liver tissues were homogenized in five volumes of buffer (210 mM mannitol, 1 mM EDTA, 70 mM sucrose, and 50 mM phosphate buffer, at pH 7.4) for measurement of antioxidant enzyme activities. The superoxide dismutase (SOD) activity and catalase (CAT) activity assays were both performed using commercial kits (Superoxide Dismutase Assay Kit and Catalase Assay Kit; Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturer’s protocols.
7
Catalase Activity Assay for A. baumannii
8
Assessing Antioxidant Defense
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To measure antioxidant defense after acute administration of test diets, plasma superoxide dismutase (SOD) and catalase were analyzed colorimetrically using superoxide dismutase assay kit (Cayman, Ann Arbor, MI, USA) and catalase assay kit (Cayman, Ann Arbor, MI, USA) according to the manufacturer’s instructions.
9
Measurement of SOD and Catalase Activities
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For the measurement of SOD activity, cells were suspended in cold HEPES buffer (20 mM HEPES pH 7.2, 1 mM EGTA, 210 mM mannitol, 70 mM sucrose), and the resulting cell lysates were centrifuged at 1500' g for 5 min at 4℃. SOD activity was measured using a SOD assay kit (Cayman Chemical Company, Ann Arbor, MI). In this assay, tetrazolium salt reacts with superoxide anion to produce formazan, which is detected at 450 nm with an ELISA microplate reader (SpectraMax190; Molecular Devices, Sunnyvale, CA).
For the measurement of catalase activity, cells were homogenized with cold phosphate buffer (50 mM potassium phosphate, pH 7.0, containing 1 mM EDTA), and the resulting cell lysates were centrifuged at 10,000'g for 15 min at 4℃. Catalase activity was determined in supernatants by the reaction of catalase with methanol in the presence of an optimal concentration of H2O2. Formaldehyde production was determined using a catalase assay kit (Cayman Chemical Company) and was quantified by measuring absorbance at 540 nm using an ELISA microplate reader (SpectraMax190, Molecular Devices).
For the measurement of catalase activity, cells were homogenized with cold phosphate buffer (50 mM potassium phosphate, pH 7.0, containing 1 mM EDTA), and the resulting cell lysates were centrifuged at 10,000'g for 15 min at 4℃. Catalase activity was determined in supernatants by the reaction of catalase with methanol in the presence of an optimal concentration of H2O2. Formaldehyde production was determined using a catalase assay kit (Cayman Chemical Company) and was quantified by measuring absorbance at 540 nm using an ELISA microplate reader (SpectraMax190, Molecular Devices).
10
Antioxidant Enzyme and Lipid Peroxidation Assays in Mouse Liver
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25 mg liver tissue was homogenized with 250 μl RIPA buffer and then centrifuged at 1600 ×g and 4°C for 10 minutes. The supernatant was collected and stored at −80°C for use. The superoxide dismutase (SOD) activity in the liver was analyzed by Randox assay kit (Cat. no. SD125, Randox Laboratories, ANT, UK) with absorbance at 340 nm. The catalase assay kit (Cat. no. 707002, Cayman Chemical, MI, USA) was used with 540 nm adsorption rate for the detection of catalase activity in the liver. For analyzing the levels of thiobarbituric acid reactive substances (TBARS) in mouse liver, 100 μl homogenized liver supernatant was mixed with 100 μl sodium dodecyl sulfate (SDS) solution and 4 ml color reagent under boiled water for an hour and then ice-cooled. The mixture was centrifuged at 1600 ×g under 4°C for 10 minutes and then the absorbance value was read at 535 nm spectrophotometrically. The data were expressed as equivalent malondialdehyde (MDA) µM/g protein [26 (link)].
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