Fsx100

Manufactured by Olympus

Sourced in Japan, United States, United Kingdom, China

The FSX100 is a high-resolution microscope designed for scientific and industrial applications. It features advanced optics and imaging capabilities to enable detailed observation and analysis of specimens. The core function of the FSX100 is to provide users with a powerful tool for magnifying and capturing images of small-scale samples.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (7 mentions) Alexa fluor 488, by Thermo Fisher Scientific (4 mentions) Triton x 100, by Merck Group (4 mentions) Cell light edu dna cell proliferation kit, by RiboBio (3 mentions) Alexa fluor 594 conjugate, by Thermo Fisher Scientific (3 mentions) Normal goat serum (ngs), by Yeasen (3 mentions) Cell light edu luminescence assay kit, by RiboBio (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (3 mentions) Alexa fluor 488 conjugated secondary antibody, by Thermo Fisher Scientific (3 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Anti f4 80, by Bio-Rad (2 mentions) Cell light edu fluorescence detection kit, by RiboBio (2 mentions) Biotinylated anti rat igg, by Vector Laboratories (2 mentions) Oct compound, by Sakura Finetek (2 mentions) 96 well plate, by BD (2 mentions) Vectashield mounting medium with dapi, by Vector Laboratories (2 mentions) Cm1950, by Leica (2 mentions) Hydromount, by National Diagnostics (2 mentions) Hematoxylin 2, by Roche (2 mentions) Discovery dab map detection kit, by Roche (2 mentions)

Close spelling variants of this product

FSX100 microscope (285 citations) FSX100 fluorescence microscope (42 citations) FSX100 Inverted Microscope (19 citations) FSX100 Bio Imaging Navigator (14 citations) FSX100 fluorescent microscope (10 citations) FSX100 imaging system (10 citations) FSX100 light microscope (8 citations) FSX100 microscopy system (7 citations) FSX100 system (7 citations) FSX100 all-in-one microscope (6 citations)

245 protocols using fsx100

Embryonic Protein Aggregation Analysis

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For histological analyses, mouse embryos were fixed in 4% paraformaldehyde (PFA), and embedded in paraffin, as described elsewhere^{6 (link)}. Sections (4-μm thick) were used for immunohistochemistry, or hematoxylin and eosin (H&E) staining. Image acquisition was performed under a fluorescence microscope (FSX100, Olympus Life Science, Waltham, MA, USA), or an optical or phase-contrast microscope, respectively.
Protein aggregation was assessed in hearts of E12.5 embryos, using the ProteoStat protein aggregation assay kits (Enzo Life Sciences, Farmingdale, NY, USA). ProteoStat emits fluorescence when it binds to the tertiary structure of aggregated proteins. It has been validated to specifically detect protein aggregates and aggresome-like inclusion bodies in cells. To label the protein aggregates, tissue sections were incubated with the ProteoStat dye for 30 min at room temperature (about 20 °C). All sections were counterstained with nuclear Hoechst (1:1000). Image acquisition was performed under a fluorescence microscope (FSX100, Olympus Life Science).

Shimizu T., Maruyama K., Kawamura T., Urade Y, & Wada Y. (2020). PERK participates in cardiac valve development via fatty acid oxidation and endocardial-mesenchymal transformation. Scientific Reports, 10, 20094.

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Zebrafish Ethanol Toxicity Study

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The lethality and teratogenicity of solvent ethanol to the zebrafish after exposure treatment for 24, 48 and 72 hours were observed with a multifunctional microscope (Olympus FSX-100, Tokyo, Japan). The dead zebrafish that did not possess heartbeat was recorded and photographed using the multifunctional microscope (Olympus FSX-100, Tokyo, Japan)with a magnification of 3.2.

Liu Y., Liu X., Wang Y., Yi C., Tian J., Liu K, & Chu J. (2019). Protective effect of lactobacillus plantarum on alcoholic liver injury and regulating of keap-Nrf2-ARE signaling pathway in zebrafish larvae. PLoS ONE, 14(9), e0222339.

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Alizarin Red Staining for Tissue Imaging

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A 0.02% wt/v Alizarin Red (Sigma-Aldrich, St. Louis, MO [A5533]) solution (pH 4.2 with NaOH) was used to stain the PAAM gel nodules, bone samples and human CAVD valves after TPEF imaging. After fixation with 4% w/v paraformaldehyde (Sigma-Aldrich, St. Louis, MO [158127]) for 10 minutes and washing with 1×PBS, the PAAM gels were placed in to the Alizarin Red solutions for 45 minutes. The same procedure was followed for the bone and human sample, but with longer fixation (20 minutes) and staining (1.5 hours) times. All samples were then washed with diH₂O several times (until the wash water was clear at the end of the rinse; about 8–10×) before imaging using an Olympus FSX100 (Tokyo, Japan).
For comparison imaging of the same fields, small portions of each sample were fixed to a glass slide with a clear adhesive. The samples were first imaged using the Leica TCS SP2 microscope and the image locations were carefully noted. The slides were then stained with Alizarin Red as described above, and imaged a second time with the Olympus FSX100 with the same fields of view that were taken with the Leica microscope described below.

Baugh L.M., Liu Z., Quinn K.P., Osseiran S., Evans C.L., Huggins G.S., Hinds P.W., Black L.D, & Georgakoudi I. (2017). Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease. Nature biomedical engineering, 1(11), 914-924.

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Quantifying Endothelial Cell Intercellular Spaces

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In vitro analysis: Endothelial cells grown on coverslips were washed and fixed with 4% paraformaldehyde, and then permeabilized in 0.1% triton-X and washed three times with PBS. Cells were then incubated with 3% BSA for 20 min followed by two washes and stained with lectin (1:1000, Vector Laboratories, CA) for 30 min, washed with PBS and mounted with Prolong antifade mounting media. Images were captured on the FSX100 Olympus microscope. Determination of intercellular spaces: lectin-stained cell cultures (3 experimental replicates) were photographed, coded and analyzed for continuity of the monolayer using a novel algorithm to determine intercellular ‘spaces’. Briefly, all images were first converted into grayscale images. A threshold was calibrated for each image to convert the grayscale image into a black and white one, such that cells are in white and everything else remains black. Thus, the total number of gaps between cells in each image was estimated by the total number of black pixels. Once the cells were identified, the two images were overlaid to find all the cells and the near empty areas in the image. The spaces near cells were calculated using the Canny edge detection algorithm. The above algorithm was coded in Python and OpenCV.

Bake S., Okoreeh A., Khosravian H, & Sohrabji F. (2018). Insulin-Like Growth Factor (IGF)-1 Treatment Stabilizes the Microvascular Cytoskeleton under Ischemic Conditions. Experimental neurology, 311, 162-172.

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Transfection of Mammalian Cells

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Cells were cultured in high‐glucose Dulbecco's modified Eagle's Medium (DMEM) medium (HyClone, Logan, UT) supplemented with 10% fetal bovine serum (FBS; HyClone) at 37°C in a 5% CO₂ standard humidified incubator. Cells in the logarithmic phase were used in all experiments. Cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific). Transfection efficiencies were validated through fluorescence microscopy (FSX100 Olympus, Tokyo, Japan).

Wang Q., Zhi Y., Ren W., Li S., Dou Z., Xing X., Quan X., Wang Y., Jiang C., Liang X., Gao L, & Zhi K. (2019). Suppression of OSCC malignancy by oral glands derived‐PIP identified by iTRAQ combined with 2D LC‐MS/MS. Journal of Cellular Physiology, 234(9), 15330-15341.

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Tissue Fixation, Sectioning, and Staining

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The animals were subjected to trans-cardiac perfusion using 4% paraformaldehyde (PFA). The tissues obtained were processed using the standard procedure and embedded into paraffin blocks, then microsectioned into 5-μm thick and placed onto glass slides. Haematoxylin-eosin (H & E) staining was used on the tissue sections and viewed using optical microscope (FSX-100 Olympus, Olympus Corporation, Shinjiku-ku, Tokyo, Japan).

Kura A.U., Cheah P.S., Hussein M.Z., Hassan Z., Tengku Azmi T.I., Hussein N.F, & Fakurazi S. (2014). Toxicity evaluation of zinc aluminium levodopa nanocomposite via oral route in repeated dose study. Nanoscale Research Letters, 9(1), 261.

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Quantification of Autophagy Dynamics Using GFP-LC3 and Chloroquine

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The GFP-LC3 puncta was quantified for the detection of autophagy. MG63 cells were transfected with GFP-LC3 (P36235, Invitrogen) for 24 h and a CQ assay was used to determine the dynamic turnover of GFP-LC3 in autolysosomes. Because CQ is a lysosome inhibitor, GFP-LC3 puncta will increase even CQ alone. However, if we could prove that GFP-LC3 puncta is increased in the Rap group treated with CQ compared to CQ alone, it would mean autophagy induced by rapamycin is not caused by the lysosome inhibitor, therefore we used CQ assay. The transfected cells were pre-treated with 50 μM CQ for 12 h. They were then washed with PBS two times and treated with or without 20 μM rapamycin for 24 h. Next, cells were fixed with 4% paraformaldehyde for 30 min. Images of individual GFP-LC3-expressing cells were taken under an epifluorescence microscope (FSX100, Olympus Optical Co.) with a 50× objective lens.

HORIE R., NAKAMURA O., YAMAGAMI Y., MORI M., NISHIMURA H., FUKUOKA N, & YAMAMOTO T. (2015). Apoptosis and antitumor effects induced by the combination of an mTOR inhibitor and an autophagy inhibitor in human osteosarcoma MG63 cells. International Journal of Oncology, 48(1), 37-44.

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Apoptosis Assay via Microscopy

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Cells were trypsinized and seeded at a density of ~1×10⁶ cells/well on 25-mm circular coverslips in 2 ml of culture medium containing 10% FBS for 24 h. Next, cells were washed with PBS and treated with 20 μM rapamycin and/or 100 μM Spautin-1 for 24 h. After treatment, cells were incubated for 15 min in a dark room with Annexin V-FITC, PI and Hoechst 33342 using the Promokine Apoptotic/Necrotic/Healthy Cells Detection kit (PromoCell GmbH, Heidelberg, Germany). Cells were then examined under an epifluorescence microscope (FSX100, Olympus Optical Co.) with a 50× objective lens.

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Visualizing Autophagy in Cultured Cells

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Cells were trypsinized and seeded at a density of ~1×10⁶ cells/well on 25-mm circular coverslips (Matsunami Glass Industries, Ltd., Osaka, Japan) in 2 ml of culture medium containing 10% FBS overnight. In the experiments testing the effect of rapamycin, cells were treated with 20 μM rapamycin for 24 h. Cells were then fixed in 4% paraformaldehyde in phosphate buffer for 30 min at room temperature and washed in phosphate-buffered saline (PBS).
For the detection of autophagy, cells were incubated with anti-LC3 antibody (code no. PM036, MBL, Nagoya, Japan) for 1 h at room temperature. Cells were then washed two times with PBS, incubated with anti-IgG secondary antibody (Alexa Fluor^® 488, code no. A11008; Invitrogen, Carlsbad, CA, USA) for 30 min at room temperature and examined under an epifluorescence microscope (FSX100, Olympus Optical Co., Ltd., Tokyo, Japan) with a 50× objective lens.

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Evaluating Cell Proliferation with EdU Assay

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An EdU assay kit (RiboBio, Guangzhou, Guangdong, China) was utilized to test DNA synthesis and cell proliferation.18 (link) Under a fluorescence microscope (FSX100, Olympus Optical Co., Ltd., Tokyo, Japan), five field-of-view were taken. The blue fluorescence was representative of total cells, and red fluorescence was reflective of the proliferating cells incorporated by EdU. The proportion of EdU positive cells to total cells was calculated.

Duan R., Li C., Wang F., Han F, & Zhu L. (2020). The Long Noncoding RNA ZFAS1 Potentiates the Development of Hepatocellular Carcinoma via the microRNA-624/MDK/ERK/JNK/P38 Signaling Pathway. OncoTargets and therapy, 13, 4431-4444.

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