Stereo discovery v20

The studied inclusion was originally immersed in a 26 × 14 × 14 mm, orange, oval Dominican amber piece with a fragmentary specimen of Psocoptera as a syninclusion, which was lost after treatment of the stone. This piece was faceted and polished for better visualization using increasingly finer sandpapers and, lastly, liquid silver polishing on a soft, clean, and dry cloth. The specimen was bought from the eBay store “ambergalleryboutique1” in July 2017. The seller confirmed that the specimen was mined in “La Toca” site. The specimen had the morphospecies code “Pheidole ufv-65” from 2017 to 2019 on Antweb.
The holotype is deposited at the Padre Jesus Santiago Moure Entomological Collection of the Universidade Federal do Paraná, Curitiba, Brazil (DZUP). Observations were made at 80× magnification with a Zeiss SteREO Discovery.V8 dissecting microscope. Measurements were made with a dual-axis micrometer stage with output in increments of 0.001 mm. All measurements are given in mm. The high-resolution images were made with an Axiocam 305 color camera coupled to a Zeiss SteREO Discovery.V20. Extended depth focus was made with Zen Blue v.2.3 and subsequently treated to correct for brightness and contrast. Digital vectorization was based on original photographs.
We adopted morphological terminology and measurements proposed by Longino (2009) and sculpture terminology by Harris (1979).

One- to seven-day-old female adults were dissected in a glass Petri dish (diameter of 90 mm) (Renyuan Company, Cangzhou, Hebei, China) containing PBS (phosphate buffer saline) buffer (Shenggong Biological Engineering Co. Ltd., Shanghai, China) using a ZSA300T Stereomicroscope (Zhong Xian Company, Chongqing, China). A tip tweezer (Shenggong Biological Engineering Co. Ltd., Shanghai, China) was used to hold the thoraco-abdominal junction of the moth, and another tip tweezer was used to remove the cephalothorax. The tweezers were then used to open the abdominal epidermis along the thoracic-abdominal junction to reveal the abdominal cavity, and to pull out the ovary and remove other tissues around the ovary [23 (link)]. The ovaries were transferred to a glass slide with a drop of PBS buffer. The glass slide was placed under a Zeiss SteREO Discovery V20 stereomicroscope (Carl Zeiss AG, Oberkochen, Baden-Württemberg, Germany) for the photographing of the ovaries. Ten females of each age were dissected.