Each bioink of interest to the present study was loaded into an EnvisionTEC Bioplotter (Gladbeck, Germany) and heated to 37 °C for 30 min (to allow for temperature equilibration prior to printing). The scaffold 3D files were designed using Autodesk Fusion 360 (San Rafael, CA); the stereolithography files were then imported into the Bioplotter RP (Version 3.0.713.1406, EnvisionTEC). Each 3D model was sliced using Bioplotter RP to the desired slice thickness based on the print nozzle used (specifically, 160, 200, and 320 µm slice thickness corresponding to the 200, 250, and 400 µm tip diameter nozzle, respectively). Once sliced, the 3D models were imported to Visual Machines (Version 2.8.115; EnvisionTEC) to be printed. All scaffolds were printed at a bioink temperature of 37 °C, 0.5–0.7 bar pressure, and at a speed of 10–12 mm/s (actual speed based on the bioink composition). A 0.3 s preflow and 0.1 s post-flow were implemented, with the printing nozzle cleaned after every three printed layers. The printing stage was kept at a temperature of 27 °C. Upon completion of the printing process, each printed scaffold was allowed to thermally set at room temperature for 5 min before removal from the printing stage. After a period of at least 72 h, the 3D-printed scaffolds were sintered using a Hot Spot 110 furnace (Zircar Zirconia, Florida, NY) to 1240 °C for a cycle of 20 h.
Bioplotter rp
Manufactured by EnvisionTEC
Sourced in Germany
The Bioplotter RP is a laboratory equipment product manufactured by EnvisionTEC. Its core function is to enable the rapid prototyping and fabrication of three-dimensional objects.
Automatically generated - may contain errors
6 protocols using bioplotter rp
1
Bioink Scaffold 3D Printing and Sintering
2
PLGA Scaffold Fabrication and Characterization
3
3D Bioplotter Fabrication Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAD models of 3D box-shaped
geometries were prepared and converted into 2D layers using BioplotterRP
(version 3.0, Envisiontec GmbH) slicer software. The slicing file
was transferred to the Visual Machine algorithm for the layer-by-layer
fabrication using the 3D Bioplotter (EnvisionTEC GmbH, Germany). The
built-in algorithms were used to produce cross-hatch printing patterns
by varying the strand diameter, the strand spacing, and the layer
slicing height, while the orientation between the layers was set at
90° (Table 6 and Figure 15 ).
Biomaterial inks were prepared and loaded into the syringe
barrel
as described under “Extrudability”. 3D constructs were
3D printed using the parameters summarized in the Supporting Information
(Table S1 ).
geometries were prepared and converted into 2D layers using BioplotterRP
(version 3.0, Envisiontec GmbH) slicer software. The slicing file
was transferred to the Visual Machine algorithm for the layer-by-layer
fabrication using the 3D Bioplotter (EnvisionTEC GmbH, Germany). The
built-in algorithms were used to produce cross-hatch printing patterns
by varying the strand diameter, the strand spacing, and the layer
slicing height, while the orientation between the layers was set at
90° (
Biomaterial inks were prepared and loaded into the syringe
barrel
as described under “Extrudability”. 3D constructs were
3D printed using the parameters summarized in the Supporting Information
(
4
Optimized 3D Printing of Poly(LA-co-CL) Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cartridge was pre-heated to a specified temperature and polymer was then added. After an interval, the temperature was changed to an optimized printing temperature: for poly(LA-co-CL), the pre-heating temperature used was 190 °C and this temperature was maintained for 5 min before printing at 175 °C.
Two different designs and shapes were printed to simulate long bone anatomy. Briefly, a 3D CAD model of each design was constructed using Magics software (EnvisionTEC). Following the instructions of the manufacturer (EnvisionTEC GmbH, Germany), the CAD models were sliced into different layers with an 80% slicing thickness of the inner diameter of the needle (ID) prior to printing. A slicing thickness of 0.32 mm (Bioplotter RP, EnvisionTEC) was used for the 0.4 mm ID stainless steel needle scaffolds, printed onto double-sided tape for better adhesion.
After printing, the scaffolds were sterilized in an inert atmosphere using electron beam radiation at a dose of 2.5 Mrad from a pulsed electron accelerator (Mikrotron, Acceleratorteknik, Stockholm, Sweden) at 6.5 MeV.
Two different designs and shapes were printed to simulate long bone anatomy. Briefly, a 3D CAD model of each design was constructed using Magics software (EnvisionTEC). Following the instructions of the manufacturer (EnvisionTEC GmbH, Germany), the CAD models were sliced into different layers with an 80% slicing thickness of the inner diameter of the needle (ID) prior to printing. A slicing thickness of 0.32 mm (Bioplotter RP, EnvisionTEC) was used for the 0.4 mm ID stainless steel needle scaffolds, printed onto double-sided tape for better adhesion.
After printing, the scaffolds were sterilized in an inert atmosphere using electron beam radiation at a dose of 2.5 Mrad from a pulsed electron accelerator (Mikrotron, Acceleratorteknik, Stockholm, Sweden) at 6.5 MeV.
5
3D Printable Electroconductive Hydrogel Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hydrogels were rendered 3-D printable by increasing the concentration of pNVP (4, 6, 8, and 12 mol%). For initial studies, only 0.1 wt% PAn-PAAMPSA was incorporated into the printable, electroconductive formulation. Electroconductive hydrogel inks were placed in 30 cc syringes with a 200-micron needle inner diameter. 3-D printing was done using an EnvisionTEC 3-D Bioplotter and accompanying software, Perfactory RP, and Bioplotter RP (EnvisionTEC GmbH, Gladbeck, Germany). The EnvisionTEC 3-D Bioplotter was housed within a custom-fabricated biosafety cabinet for BSL-2 printing of human cells. Hydrogels were printed at a z-offset of 0.12 mm, a printing speed of 8.0 mm/s, and an extrusion pressure of 0.8 bar. The process of fabrication is shown in Figure 1 B. The 3-D constructs were printed layer-by-layer, with UV light exposures for 30 s between layers. Following crosslinking, hydrogels were hydrated using the same protocol used for photopatterned hydrogels.
6
Osteochondral Interfacial Shear Strength
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!