Rabbit anti mouse igg peroxidase

The antibodies with their dilutions and sources were as follows: antibodies for immunofluorescence: mouse monoclonal anti‐HA‐tag [1 : 200; Cell Signaling Technologies (CST; Danvers, MA, USA)], rabbit polyclonal anti‐calnexin (CANX, 1 : 200; Santa Cruz Biotechnology, Dallas, TX, USA), Alexa Fluor 568‐goat anti‐mouse IgG (1 : 200; Molecular Probes, Eugene, OR, USA), Alexa Fluor 647‐goat anti‐rabbit IgG (1 : 200; Molecular Probes). Antibodies for western blotting: rabbit polyclonal anti‐HA (1 : 4000; Sigma‐Aldrich, St. Louis, MO, USA), anti‐Histone H3(1 : 1000, CST), rabbit polyclonal anti‐GAPDH (1 : 2500; Abcam, Cambridge, UK), mouse monoclonal anti‐α‐tubulin (1 : 10 000; Sigma‐Aldrich), rabbit anti‐ CANX (1 : 1000; CST), rabbit anti‐BiP (1 : 1000, CST), rabbit anti‐GRP94 (1 : 1000, CST), mouse anti‐ERP72 (1 : 200; Santa Cruz Biotechnology), goat anti‐SEL1L (1 : 200; Santa Cruz Biotechnology), rabbit anti‐HRD1 (1 : 500; CST), rabbit anti‐OS‐9 (1 : 500; Abcam), goat anti‐rabbit IgG‐peroxidase (1 : 50 000; Sigma‐Aldrich), and rabbit anti‐mouse IgG‐peroxidase (1 : 80 000; Sigma‐Aldrich).

The antibodies with their dilutions and sources were as follows: Antibodies for western blotting: rabbit polyclonal anti-HA (1:4000; H6908, Sigma, Lot No: 022M4806), mouse monoclonal anti-α-tubulin (1:10,000; Sigma, T5168, Lot No: 103M4773V), goat anti-SEL1L (1: 200; Santa Cruz Biotechnology, SC-48081, Lot No: C3109), Rabbit anti-HRD1 (1:500: Cell Signaling technology, 12925 S, Lot No: 1), rabbit anti-OS-9 (1: 500: Abcam, ab19853, Lot No: GR54041-1), rabbit anti-Calnexin (1:1000; Cell Signaling Technology, 2433 S, Lot No: 2), mouse monoclonal anti-ubiquitin (1:1000; Sigma, U0508, Lot No: SLBL1928V), Rabbit anti-Histone-H3 (1:1000; Cell Signaling Technology, 9715S, Lot No: 18), Rabbit anti-GAPDH (1: 2500; Abcam, ab9485, Lot No: GR184357-1), Rabbit anti-LC3-B (1: 1000; Sigma, L7543, Lot No: 046M4787V), goat anti-rabbit IgG-peroxidase (1: 50,000; Sigma), rabbit anti-mouse IgG-peroxidase (1:80,000; Sigma), chicken anti-goat IgG-peroxidase (1:5000, Santa Cruz Biotechnology).

The following peptides corresponding to A349-K372 of the human β2AR were synthesized by United BioSystems (Herdon, VA): A349-K372 (AYGNGYSSNGNTGE QSGYHVEQEK), A349-K372pS355 (AYGNGY (pS) SNGNTGEQSGYHVEQEK), and A349-K372pS356 (AYGNGYS (pS) NGNTGEQSGYHVEQEK). Mouse monoclonal antibodies against phospho-S262 peptides were a gift from Dr. Richard B. Clark (The University of Texas, Houston). Rabbit anti-β2AR, rabbit anti-phospho-serine 345/346, and rabbit anti-phospho-serine 355/356 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). Rabbit anti-mouse IgG-peroxidase labeled was purchased from Sigma (St. Louis, MO). Goat anti-rabbit IgG-peroxidase labeled was purchased from KPL (Gaithersburg, MD).