Isolute sle column

Manufactured by Biotage

Sourced in Sweden

Isolute® SLE + columns are liquid-liquid extraction (LLE) sample preparation devices designed for the isolation and purification of analytes from complex biological matrices. They utilize a supported liquid extraction (SLE) technique to facilitate efficient extraction and clean-up of samples prior to analysis.

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Lab products found in correlation

13c3 androstenedione, by Merck Group (1 mentions) 13c3 testosterone, by Merck Group (1 mentions) 13c3 progesterone, by Merck Group (1 mentions) Aea d4, by Cayman Chemical (1 mentions) 2 ag d5, by Cayman Chemical (1 mentions) Oea d4, by Cayman Chemical (1 mentions) Pea d4, by Cayman Chemical (1 mentions) Chromasolv, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Ammonium fluoride, by Merck Group (1 mentions) Morphine, by Cerilliant (1 mentions) Cotinine, by Cerilliant (1 mentions) Sertraline, by Cerilliant (1 mentions) Clonazepam, by Cerilliant (1 mentions) Dihydrocodeine, by Cerilliant (1 mentions) Caffeine, by Cerilliant (1 mentions) Tert butyl methyl ether, by Thermo Fisher Scientific (1 mentions) Cocaine d3, by Cerilliant (1 mentions) Pressure 48 positive pressure manifold, by Biotage (1 mentions)

Spelling variants of this product

ISOLUTE® supported liquid extraction (SLE+) columns (4 citations) ISOLUTE® SLE+; (3 citations) Isolute SLE+ column cartridges (2 citations)

4 protocols using isolute sle column

Quantification of Endocannabinoids and Metabolites

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OEA, PEA, the deuterated standards D₉-progesterone and D₇-cortisone, and ¹³C-labeled standards ¹³C₃-androstenedione, ¹³C₃-testosterone, and ¹³C₃-progesterone were purchased from Sigma-Aldrich (Buchs, Switzerland). ¹³C₃-cortisol and ¹³C₃-cortisone were purchased from Isoscience (Ambler, USA). AEA, 2-AG, and the deuterated eCBs (D₄-AEA, D₅-2-AG, D₄-OEA, D₄-PEA, and D₁₁-AEA) were purchased from Cayman Chemicals (Ann Arbor, USA). Water and methanol (MeOH) were of LC–MS grade (Chromasolv®) and purchased from Sigma-Aldrich (Buchs SG, Switzerland). Acetone, ethyl acetate, and ammonium fluoride were purchased from Merck (Darmstadt, Germany). Reconstitution solution consisted of 0.2 mM NH₄F in water/methanol 97/3 v/v, respectively. Isolute® SLE + columns were purchased from Biotage® (Uppsala, Sweden). All chemicals were of highest analytical grade.

Restin T., Byland N., Voegel C.D., La Marca-Ghaemmaghami P., Baumgartner M.R., Bassler D., Kraemer T, & Binz T.M. (2022). Endocannabinoid and steroid analysis in infant and adult nails by LC–MS/MS. Analytical and Bioanalytical Chemistry, 414(20), 6201-6211.

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Optimized LC-MS Workflow for DOA

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Optima LC/MS grade methanol (MeOH), LC/MS grade 0.1% formic acid in water, LC/MS grade formic acid ampules, optima grade dichloromethane (DCM), optima LC/MS grade isopropanol (IPA), hydrochloric acid HCl, tert-butyl methyl ether, (MTBE), and hexane were purchased from Fisher Scientific. Ammonium hydroxide (NH4OH was purchased from Sigma. DOA analytical standards: (+/-) amphetamine, (+/-) methamphetamine, cocaine, benzoylecgonine, morphine, phencyclidine, (-) delta9-THC, (+/-) amphetamine -D5, (+/-) methamphetamine -D5, cocaine -D3, benzoylecgonine -D3, morphine -D3, phencyclidine -D5, and (-) delta9-THC -D3 were obtained as 1mg/mL solutions from Cerilliant (Round Rock, TX, USA). Interfering substances: Diphenhydramine HCl, Alprazolam, Dihydrocodeine, S-Nicotine, Caffeine, Cortisol, -Cotinine, Dextromethorphan, Ibuprofen, Naproxen, Prednisone, R-Pseudoephedrine, Amobarbital, Propranolol, Nicotinamide, Carbamazepine, Clobazam, Clonazepam, Valproic Acid, Verapamil, and Sertraline were purchased from Cerilliant (Round Rock, TX, USA). Stabilization buffer was purchased from Immunalysis (Pomona, CA). The Raptor Bipheyl LC column; 2.7um, 100mm x 2.1mm was purchased from Restek (Bellefonte, PA). Isolute SLE+ columns were purchased from Biotage (Charlotte, NC). The PRESSURE+ 48 positive pressure manifold was obtained from Biotage (Charlotte, NC).

Howard M.K., Russo P., Haymond A., Ortiz V.C., Andes S.R., Espina V., Luchini A., Liotta L.A., & Guirguis R. (2020). LC-MS/MS Validation for Drug of Abuse Testing Utilizing a Split Sample Oral Fluid Collection System.

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Serum Free Fatty Acid Extraction

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We added 0.3 mL of PBS containing internal standards (100 ng of margaric acid) was added to 20 μL of serum, and the sample was mixed with a vortex. FFAs were extracted using an ISOLUTE SLE+ column (Biotage) and dichloromethane. The lower organic region of the sample was collected and dried under nitrogen stream. The residue was dissolved in 5 μL of pyridine and 30 μL of the reagent BSTFA+TMCS (99:1; TS-38831, Thermo Fisher Scientific) for trimethylsilylation. The derivatization reaction was performed for 30 minutes at 40°C.

Katoh K., Katoh Y., Kubo A., Iida M., Ikeda Y., Iwata T., Nishio H., Sugawara M., Kato D., Suematsu M., Hirai S, & Kawana K. (2023). Serum Free Fatty Acid Changes Caused by High Expression of Stearoyl-CoA Desaturase 1 in Tumor Tissues Are Early Diagnostic Markers for Ovarian Cancer. Cancer Research Communications, 3(9), 1840-1852.

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Quantification of Urinary NNAL by UPLC-MS/MS

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Urinary total NNAL was quantified following a published procedure (12 (link)). Briefly, urine samples (250 μL)
with 2 pmols of ¹³C₆-NNAL were incubated with 3000 units
of β-glucuronidase enzyme (in 50 μL PBS) overnight at 37°.
After incubation, the samples were subjected to primary and secondary extraction
using Isolute SLE+ column (Biotage, Part # 820–0140-C) and MCX 1cc (30mg)
Extraction Cartridge (Waters, Part # 186000252). The eluted samples were dried
under speed-vac and dissolved in 10mM ammonium acetate (30 μL) and
analyzed by UPLC-MS/MS.

Wang Y., Narayanapillai S.C., Tessier K.M., Strayer L.G., Upadhyaya P., Hu Q., Kingston R., Salloum R.G., Lu J., Hecht S.S., Hatsukami D.K., Fujioka N, & Xing C. (2020). The Impact of One-week Dietary Supplementation with Kava on Biomarkers of Tobacco Use and Nitrosamine-based Carcinogenesis Risk among Active Smokers. Cancer prevention research (Philadelphia, Pa.), 13(5), 483-492.

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