N n n n tetramethylethylenediamine

Manufactured by Sinopharm

Sourced in China

N,N,N′,N′-tetramethylethylenediamine is a chemical compound that functions as a buffer and catalyst. It is commonly used in various laboratory applications.

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Lab products found in correlation

5 protocols using n n n n tetramethylethylenediamine

Characterization of Shrimp Spoilage Bacteria

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The shrimp (L. vannamei) was bought from Qidong Rd. Market, Qingdao, with a weight of 45 ± 5 g. The shrimp was transported to the laboratory on ice within 30 min.
The wild strain of L. plantarum SS-128 and the luxS-mutant strain of L. plantarum SS-128 (ΔluxS) were constructed and preserved by the Laboratory of Aquatic Products Higher Application Technology, Ocean University of China. Shewanella baltica is a laboratory-preserved strain previously isolated from spoiled L. vannamei (Zhu et al., 2015 (link)). DPD was obtained from Professor Ming Li’s lab in the College of Pharmacy, Ocean University of China. The De Man, Rogosa and Sharpe (MRS) broth, Lactobacillus medium, and agar were bought from Qingdao Haibo Biotechnology Co., Ltd., China. Na₂HPO₄⋅12H₂O, NaH₂POx⋅H₂O, NaCl, 40% Acr-Bis, Tris–HCl, N,N,N′,N′-tetramethylethylenediamine, Tris, dithiothreitol, and other analytical pure chemical reagents were purchased from Sinopharm Group Chemical Reagent Co., Ltd.

Li Y., Qian Y., Lou X., Hu Z., Hu Y., Zeng M, & Liu Z. (2022). LuxS in Lactobacillus plantarum SS-128 Improves the Texture of Refrigerated Litopenaeus vannamei: Mechanism Exploration Using a Proteomics Approach. Frontiers in Microbiology, 13, 892788.

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Fabrication of Porous Hydrogel Scaffolds

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Example 2

Materials: m-acrylamido phenylboronic acid, and the structure was as follow:

[Figure (not displayed)]

N-[Tris(hydroxymethyl)methyl]acrylamide was purchased from Alfa Aesar, and the structure as follow:

[Figure (not displayed)]

Acrylamide (AAm) was purchased from J&K. Ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine were purchased from Sinopharm. Diphenyliodonium nitrate was purchased from Aladdin.

Preparation: 446 mg acrylamide, 60 mg m-acrylamido phenylboronic acid and 55 mg N-[Tris(hydroxymethyl)methyl]acrylamide were dissolved in the 5 ml deionized water. After fully dissolved, 100 μL Ammonium persulfate and 10 μL N,N,N′,N′-tetramethylethylenediamine were added into the solution. The solution was transferred to a sealed glass vessel quickly. 24 hours later, the resulting gel was immersed in a solution which contains the diphenyliodonium nitrate (control the pH=10) for 24 hours. The gel was frozen by a cooling stage. Under the freezing condition, the gel was exposed by UV irradiation integrally for 5 min. After thawing, the gel swelled in the water to obtain porous gels. The laser scanning confocal microscope was applied to characterize the pore structure of the porous gel.

US10358516B2. Method for preparing porous hydrogel through freezing-illumination (2019-07-23). ZHEJIANG UNIVERSITY [CN]. Inventors: Qian Zhao [CN], Jingjun Wu [CN], Tao Xie [CN], Di Chen [CN].

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Shrimp Probiotic and Quorum Sensing Modulation

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Shrimp (Litopenaeus vannamei) weighing 45 ± 5 g were purchased from the local fishery market at Qidong Rd., Qingdao, and were transported to the laboratory on ice within half an hour.
Lactobacillus plantarum SS-128 (L. plantarum SS-128) was extracted from the intestine of blackhead fish and preserved by the Laboratory of Aquatic Products Higher Application Technology, Ocean University of China. The luxS-mutant strain of Lactobacillus plantarum SS-128 (L. plantarum ΔluxS) was constructed previously in the lab. Shewanella baltica A02 (S. baltica A02) was isolated from spoiled shrimp. Pro donated the 4,5-dihydroxy-2,3-pentanedione (DPD),Ming Li’s lab in the College of Pharmacy, Ocean University of China. The MRS broth, agar, and Lactobacillus medium were purchased from Qingdao Haibo Biotechnology Co., Ltd., China. The NaCl, N,N,N’,N’-tetramethylethylenediamine, Na₂HPO₄·12H₂O, and other analytical pure chemical reagents were purchased from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China.

Li Y., Liu T., Meng X., Qian Y., Yan S, & Liu Z. (2022). AI-2/Lux-S Quorum Sensing of Lactobacillus plantarum SS-128 Prolongs the Shelf Life of Shrimp (Litopenaeus vannamei): From Myofibril Simulation to Practical Application. Foods, 11(15), 2273.

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Synthesis and Characterization of Hydrogel Composites

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CMC (CP, viscosity: 300-800 mpa·s) and AM (CP) were purchased from Shanpu Chemical Factory (Shanghai, China) and used without further treatment. Ammonium persulfate (APS, AR), N,N,N'N'-tetramethyl ethylenediamine (TMEDA, AR), Methyl violet (MV), and Methylene blue (MB) were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). ATP was obtained from Huangnishan Mine in Xuyi county, Jiangsu province, China, and then ground and passed through a 200-mesh sieve. N,N'-methylenebisacrylamide (MBA, CP) was received from Yuanfan Vegetable Additives (Shanghai, China). T-20 (AR) was received from BASF Corporation. 3A molecular sieve 103 was obtained from Molsion Molecular Sieve Co., Ltd. (Shanghai, China). Linseed oil was purchased from Yongfan Trading Co. (Lanzhou, China). Colza oil and soybean oil were purchased from Yihaijiali Arawana Edible Oil Co., China. Peanut oil, sunflower oil, olive oil, and sesame oil were obtained from Shandong Luhua Group Co., Ltd. China. Corn oil was purchased from Cofco Food Marketing Co., Ltd. China. Other reagents were all of analytical grade, and all solutions were prepared with deionized water.

Wang F., Zhu Y, & Wang A. (2020). Preparation of Carboxymethyl Cellulose-g- Poly(acrylamide)/Attapulgite Porous Monolith With an Eco-Friendly Pickering-MIPE Template for Ce(III) and Gd(III) Adsorption. Frontiers in Chemistry, 8, 398.

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Purification and Analysis of Paclitaxel

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Semen Armeniacae Amarum (SA) was purchased from Beijing Yanjing Chinese Medicinal Herbs Co. Ltd. (China). BCA kit was purchased from Nanjing Jiancheng Bioengineering Institute (China). High Q ion exchange gel was purchased from Bio-Red Laboratories (USA). The TSK-GEL G6000 PW column was purchased from TOSOH (Japan). β-MercaptoEthanol was purchased from Amresco Co. (USA).
Paclitaxel (99.5% purity), sodium dodecyl sulphate, glycine and Trizma base were purchased from Sigma Chemical Co. (USA). Ethanol, acetone, carbamide, hydrochloric acid, glacial acetic acid, sodium chloride, N,N,N',N'-Tetramethyl ethylene diamine, tween 20, bromophenol blue, ammonium persulphate and EDTA were purchased from Sinopharm Chemical Reagent Co., Ltd (China).

Lin D., Lin W., Gao G., Zhou J., Chen T., Ke L., Rao P, & Wang Q. (2020). Purification and characterization of the major protein isolated from Semen Armeniacae Amarum and the properties of its thermally induced nanoparticles. International journal of biological macromolecules, 159.

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