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Methyl tricosanate

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Methyl tricosanate is a long-chain fatty acid methyl ester. It is used as a laboratory standard and reference material in analytical chemistry.

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Lab products found in correlation

Acetyl chloride, by Merck Group (3 mentions) Methyl heptadecanoate, by Merck Group (3 mentions) 3 5 di tert butyl 4 hydroxytoluene bht, by Merck Group (3 mentions) Hexane, by Sinopharm (2 mentions) Methanol, by Sinopharm (2 mentions) K2co3, by Sinopharm (2 mentions) Nah2po4 2h2o, by Sinopharm (1 mentions) Sodium 3 trimethylsilyl 2 2 3 3 2h4 propionate tsp, by Cambridge Isotopes (1 mentions) K2hpo4 3h2o, by Sinopharm (1 mentions)

3 protocols using methyl tricosanate

1

Fatty Acid Methyl Ester Analysis

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Deuterium oxide (D2O, 99.9% D) was obtained from Cambridge Isotope Laboratories, Inc. (Miami, USA.). Methanol, hexane, and K2CO3 were obtained all in analytical grade from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Methyl heptadecanoate, methyl tricosanate, and acetyl chloride (99.0%) were purchased from Sigma-Aldrich (St. Louis, MO) whereas 3,5-Di-tert-butyl–4-hydroxytoluene (BHT) and a mixed standard methyl esters of 37 fatty acids were obtained from Supelco (Bellefonte, PA).
Tian Y., Xu T., Huang J., Zhang L., Xu S., Xiong B., Wang Y, & Tang H. (2016). Tissue Metabonomic Phenotyping for Diagnosis and Prognosis of Human Colorectal Cancer. Scientific Reports, 6, 20790.
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2

Fatty Acid Profiling by NMR Spectroscopy

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Deuterium oxide (D2O, 99.9% D) and sodium 3-(trimethylsilyl) [2,2,3,3-2H4] propionate (TSP) were purchased from Cambridge Isotope Laboratories, Inc. (Miami, U.S.A.). Methanol, hexane, K2CO3, K2HPO4·3H2O, and NaH2PO4·2H2O (all in analytical grade) were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Methyl heptadecanoate, methyl tricosanate, and acetyl chloride (99.0%) were bought from Sigma-Aldrich (St. Louis, MO). 3,5-Di-tert-butyl-4-hydroxytoluene (BHT) and a mixed standard methyl esters of 37 fatty acids were obtained from Supelco (Bellefonte, PA). A buffer containing 0.001% TSP and 80% D2O was prepared with K2HPO4 and NaH2PO4 (0.15 M, pH 7.43) and used as solvent for NMR analysis of tissue extracts.
Tian Y., Nie X., Xu S., Li Y., Huang T., Tang H, & Wang Y. (2015). Integrative metabonomics as potential method for diagnosis of thyroid malignancy. Scientific Reports, 5, 14869.
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3

Hemolymph NMR Analysis Protocol

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Analytical grade thiourea, NaH2PO4·2H2O, K2HPO4·3H2O, hexane, K2CO3, and NaCl were obtained from Shanghai Sinopharm Chemical Reagent Co. Ltd. (China). Sodium azide (NaN3) was from Tianjin FuChen company (China). Methyl tricosanate (99.0%), methyl heptadecanoate, acetyl chloride, and D2O (99.9% D) were bought from Sigma-Aldrich. A mixed standard consisting of methyl esters of 37 fatty acids and 3,5-di-tert-butyl-4-hydroxytoluene (BHT) were obtained from Supelco (Bellefonte, PA). Phosphate buffer (0.045M, pH7.41) was prepared from NaH2PO4 and K2HPO4 containing 50% D2O and used as adding solvent for NMR analysis of hemolymph samples with good low-temperature stability of this buffer.40 (link)
Zhou L., Li H., Hao F., Li N., Liu X., Wang G., Wang Y, & Tang H. (2015). Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx moriL.). Journal of proteome research, 14(5), 2331-2347.
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