β actin clone d6a8

Manufactured by Cell Signaling Technology

β-actin (clone D6A8) is a primary antibody recognizing the β-actin protein, a widely expressed cytoskeletal protein involved in various cellular processes. This antibody can be used to detect the expression of β-actin in a variety of sample types.

Automatically generated - may contain errors

Lab products found in correlation

Pvdf membrane, by GE Healthcare (2 mentions) Ripa lysis buffer, by Merck Group (2 mentions) Tween 20, by Merck Group (2 mentions) Restore western blot stripping buffer, by Thermo Fisher Scientific (2 mentions) Thepierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Bovinealbumin fraction 5, by MP Biomedicals (2 mentions) Phospho p44 p42 mapk erk1 2 thr202 thy204, by Cell Signaling Technology (2 mentions) Pax8 clone pax8r1, by Abcam (2 mentions) E cadherin clone 24e10, by Cell Signaling Technology (2 mentions) Complete mini edta free, by Roche (2 mentions) α tubulin clone dm1a, by Santa Cruz Biotechnology (2 mentions) Cell lytic m, by Merck Group (1 mentions) Trans blot system, by Bio-Rad (1 mentions) Novex 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Pax8 10336 1 ap, by Proteintech (1 mentions) Hsp90 clone c45g5, by Cell Signaling Technology (1 mentions) C myc clone y69, by Abcam (1 mentions) Antibody against tfe3 hpa023881, by Merck Group (1 mentions)

Spelling variants of this product

β-actin (6244 citations) β-actin (D6A8) (15 citations) Actin (D6A8) (3 citations) Anti-β-actin (clone D6A8) (2 citations)

4 protocols using β actin clone d6a8

Protein Expression Analysis in Mouse Thyroid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SPTL and mouse thyroid follicular cells were lysed with RIPA Lysis Buffer
(MilliporeSigma) with proteinase inhibitor cocktail, cOmplete mini EDTA-Free (Roche). The
Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used for measuring the protein
concentration of lysates. Samples were subjected to SDS-PAGE and transferred to PVDF
membranes (GE Healthcare). Tris-buffered saline (TBS) buffer containing 5% bovine
albumin fraction V (MP Biomedicals) and 0.1% Tween-20 (Sigma-Aldrich) was used for
blocking. Stripping of membrane was performed using Restore Western Blot Stripping Buffer
(Thermo Fisher Scientific). Antibodies used were those against AKT (#9272, Cell
Signaling Technology), phospho-p44/p42 MAPK (ERK1/2) (Thr202/Thy204) (#9101, Cell
Signaling Technology), ERK1+ERK2 (ab17942, Abcam), PAX8 (clone PAX8R1, ab53490, Abcam),
E-cadherin (clone 24E10, Cell Signaling Technology), α-tubulin (clone DM1A,
sc-32293, Santa Cruz), and β-actin (clone D6A8, #8457, Cell Signaling
Technology). Anti-NKX2-1 and anti-phospho-AKT (Ser473) antibodies were the same as those
used for immunohistochemistry/immunofluorescence.

Iwadate M., Takizawa Y., Shirai Y.T, & Kimura S. (2018). An in vivo model for thyroid regeneration and folliculogenesis. Laboratory investigation; a journal of technical methods and pathology, 98(9), 1126-1132.

+ Open protocol

+ Expand

Molecular Characterization of LVRCC67 Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols

For validation of gene deletions and kidney cell origin of LVRCC67 cell line, whole cell extracts were prepared using CellLytic M (Sigma) and sonication for 10mins in a 4°c water bath. After centrifugation at 21,000rpm, supernatants were mixed with 6x loading dye containing 2-mercaptoethanol and run on pre-cast Novex 4–12% Bis-Tris gels (Invitrogen) for 90mins at 130V using the BioRad Transblot system. Proteins were transferred onto methanol-activated PVDF membranes for 1hour using 100V. Membranes were blocked with 5% milk and incubated at 4°c overnight in 5% BSA containing primary antibodies (Pax8 #10336-1-AP Proteintech 1:2500, HIF-1ɑ clone D1S7W Cell Signaling Technology 1:1000, c-myc clone Y69 Abcam 1:1000, Rb1 #9301S Cell signaling Technology 1:500, p53 clone 1C12 Cell Signaling Technology 1:1000, CD10 #PA5-47075 Invitrogen 1:2000, HSP-90 clone C45G5 Cell signaling Technology 1:3000, β-actin clone D6A8 1:5000) followed by secondary antibody incubation the following day for 1hour at room temperature.

Rappold P.M., Vuong L., Leibold J., Chakiryan N.H., Curry M., Kuo F., Sabio E., Jiang H., Nixon B.G., Liu M., Berglund A.E., Silagy A.W., Mascareno A., Golkaram M., Marker M., Reising A., Savchenko A., Millholland J., Chen Y.B., Russo P., Coleman J., Reznik E., Manley B.J., Ostrovnaya I., Makarov V., DiNatale R.G., Blum K.A., Ma X., Chowell D., Li M.O., Solit D.B., Lowe S.W., Chan T.A., Motzer R.J., Voss M.H, & Hakimi A.A. (2022). A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma. Cancer discovery, 12(10), 2308-2329.

+ Open protocol

+ Expand

Protein Expression Analysis in Mouse Thyroid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Antibody Panel for Autophagy and mTOR Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

NMT1 antibody was from Novus Biologicals (NBP2-32168) and NMT2 antibody from BD Transduction (611310). Antibodies against LC3B (clone D11), cleaved caspase-3 (9661), mTOR (clone 7C10), LAMTOR1 (clone D11H6), phospho-p70 S6 Kinase (Thr389) (9205), p70 S6 kinase (clone 49D7), phospho-4E-BP1 (Thr37/46), 4E-BP1 (clone 53H11), and β-Actin (clone D6A8) were from Cell Signaling Technology. p62^SQSTM antibody (18420-1-AP) was from ProteinTech. Antibody against TFE3 (HPA023881) was from Sigma Life Science. Antibody against LAMP1 (clone H4A3) to was from SouthernBiotech, and the antibody against GAPDH (clone 6C5) was from Millipore Sigma.

Chen Y.C., Navarrete M.S., Wang Y., McClintock N.C., Sakurai R., Wang F., Chen K.T., Chou T.F., Rehan V.K., Lee D.J, & Diaz B. (2020). N-myristoyltransferase-1 is necessary for lysosomal degradation and mTORC1 activation in cancer cells. Scientific Reports, 10, 11952.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!