In brief, the quantities of protein in samples extracted from cardiomyocytes or the peri‐infarct region of mice were determined using a bicinchoninic acid kit. Equivalent amounts of protein were loaded in equal volumes and fractionated by SDS‐PAGE (10–15% polyacrylamide gels). GAPDH was used as the internal control. The antibodies were as follows: anti‐TGFβR3, anti‐phospho‐p38, anti‐phospho‐ERK1/2, anti‐phospho‐JNK1/2, anti‐p38, anti‐ERK1/2, anti‐JNK1/2 (1:1000 dilution; Cell Signaling Technology), and anti‐GAPDH (1:500 dilutions; Research Diagnostics).
Anti tgfβr3
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
Anti‐TGFβR3 is a laboratory reagent that detects the transforming growth factor beta receptor III (TGFβR3) protein. TGFβR3 is a transmembrane protein that functions as a co-receptor for the TGF-beta signaling pathway. This product can be used to identify and quantify TGFβR3 in various biological samples through techniques such as Western blotting, immunohistochemistry, or flow cytometry.
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Lab products found in correlation
Anti phospho jnk1 2, by Cell Signaling Technology (1 mentions)
Anti jnk1 2, by Cell Signaling Technology (1 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti tgfβr2, by Abcam (1 mentions)
Goat anti rabbit igg conjugated to horseradish peroxidase, by Thermo Fisher Scientific (1 mentions)
Anti total smad2 3, by Cell Signaling Technology (1 mentions)
Anti tgfβr1, by Santa Cruz Biotechnology (1 mentions)
Anti phospho smad2, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti tgfβr3
1
Protein Expression Analysis in Cardiac Tissue
2
Western Blot Analysis of TGFβ Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein extracts were prepared from mouse lung tissue and cultured cells as described previously (16) . The following antibodies were employed: anti-TGFβR1 (Santa Cruz Biotechnology, Santa Cruz, CA; 1 : 1,000); anti-TGFβR2 (Abcam, Cambridge, UK; 1 : 500); and anti-TGFβR3 (1 : 100), anti-total-Smad2/3 (1 : 1,000), anti-phospho-Smad2 (phospho-Smad2 Ser465/467; 1 : 1,000), anti-β-actin (1 : 1,000) (all from Cell Signaling, Cambridge, UK). Immune complexes were detected with goat-antirabbit IgG conjugated to horseradish peroxidase (ThermoScientific, Waltham, MA; 1 : 6,000) by enhanced chemiluminescence (SuperSignalWest Femto Maximum Sensitivity Substrate; ThermoScientific).
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